塞内加尔高粱幼苗对5型弧菌的反应

GM crops Pub Date : 2022-05-02 DOI:10.3390/crops2020011
E. Ahn, L. Prom, C. Fall, C. Magill
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引用次数: 5

摘要

高粱的头黑穗病是由弧菌引起的。采用3种不同的处理方法对36份塞内加尔高粱材料进行了评价,这些材料由尚未对5型弧菌有反应的高粱品系组成。首先,在琼脂中接种端孢子,在土壤中接种幼苗,然后在接种后4 d浸入水中。浸没6天后,在第一片叶子上观察到感染的迹象(明显的斑点)。其次,将浸渍了端孢子的琼脂置于盆中,围绕茎部接种同一时期的幼苗,移入温室,生长至穗全发育阶段。第三,在温室内采用注射器接种方式接种幼苗。虽然温室内土壤接种的幼苗在穗外露时没有出现症状,因此没有发生全身性感染,但在温室内注射器接种时,88.9%的受试品种出现全身性感染。根据先前的研究,在水下接种的幼苗导致广泛的明显斑点,这些斑点被认为是潜在的感染位点。此外,幼苗接种导致几丁质酶和PR10的表达略有上调,这些基因与植物地上部分的防御相关。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Response of Senegalese Sorghum Seedlings to Pathotype 5 of Sporisorium reilianum
Sporisorium reilianum causes head smut in sorghum. A total of 36 Senegalese sorghum accessions comprised of sorghum lines that have not been explored with response to pathotype 5 of S. reilianum were evaluated with 3 different treatments. First, seedling shoots were inoculated while still in soil with teliospores in agar, and then submerged under water at 4 days post inoculation. Signs of infection (noticeable spots) on the first leaf were observed up to 6 days post submergence. Second, seedlings at the same stage were inoculated by placing the teliospore impregnated agar around the stem in pots, moved to a greenhouse and grown to full panicle development stage. Third, seedings were inoculated via syringe inoculation in the greenhouse. Although soil inoculated seedlings grown in the greenhouse did not result in systemic infection as determined by lack of symptoms at panicle exsertion, 88.9% of tested cultivars showed systemic infections when syringe inoculated in the greenhouse. Inoculation of seedlings maintained under water led to broad range of noticeable spots that are assumed to be potential infection sites based on a previous study. In addition, seedling inoculation led to slightly upregulated expression of chitinase and PR10, genes that are associated with defense in aerial parts of plants.
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