[2-14C]醋酸酯在仔猪肝脏和肠黏膜合成脂质的特点

O. Zakhariv, I. Vudmaska, A. Petruk
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引用次数: 2

摘要

哺乳仔猪对脂肪的需要量有两种供应方式:母猪乳和从头合成。尽管牛奶中的脂肪含量很高,脂肪生成也增加了,但仔猪在出生后的第一个月体内的脂肪含量增加非常轻微,这与这一时期的极快生长有关,因此,用于组织形成的能量和结构脂的消耗很大。因此,研究哺乳仔猪脂质合成强度具有重要意义。我们研究的目的是研究与年龄相关的脂肪生成动力学。选取12头大型白种母猪。每头母猪分别在1日龄、10日龄和30日龄各取3头仔猪。仔猪肌肉注射剂量为100 μCi的[2-14C]醋酸钠水溶液。2 h后处死仔猪,取肝、小肠和大肠黏膜标本。组织均质化;用薄层色谱法提取脂类并进行分类。每个部分的放射性是由闪烁计数器测定的。结果表明:1日龄仔猪肝脏中[2-14C]醋酸酯合成脂的强度是10日龄和30日龄时的2.5倍;1日龄和10日龄仔猪小肠粘膜和大肠粘膜的脂质合成强度几乎相同。在30日龄时,小肠黏膜中含量显著增加,大肠黏膜中含量显著降低。与肝脏相比,研究各阶段仔猪小肠黏膜各脂类合成中[2-14C]乙酸的使用程度存在显著差异。10日龄和30日龄仔猪大肠黏膜中各类脂类的合成也具有相同的特征。结果表明,仔猪小肠、大肠黏膜和肝脏的脂质合成存在显著差异。在仔猪生命的前30天,乙酸被大量用于脂质合成。在出生的第一天,脂肪生成在肝脏中最活跃,但随着年龄的增长,脂肪合成逐渐在肠粘膜中激活。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Features of lipid synthesis from [2-14C] acetate in liver and intestinal mucosa of piglets
Requirement of suckling piglets in lipids are supplied in two ways: with sow milk and by de novo synthesis. Despite the high fat content of milk and increased lipogenesis, lipid content in piglets’ body during the first month of life increases very slightly what is associated with extremely rapid growth at this period and, consequently, significant expenditure of energy and structural lipids for tissues formation. Therefore, it is important to study the intensity of lipid synthesis in suckling piglets. The purpose of our studies was to investigate the age-related dynamics of lipogenesis. Twelve sows of large white breed were selected. From each sow, three piglets were taken at 1-, 10- and 30-day-old age. The piglets were intramuscularly injected with an aqueous solution of [2-14C] sodium acetate at a dose of 100 μCi. After 2 hours, the piglets were killed and samples of the liver, small and large intestine mucosa were obtained. The tissues were homogenized; lipids were extracted and divided into classes by thin layer chromatography. The radioactivity of each fraction was determined by a scintillation counter. The results show that the intensity of lipid synthesis from [2-14C] acetate in liver of 1 day-age piglets was 2.5 times higher than at 10 and 30 days. In the mucous membrane of the small and large intestine of piglets at 1- and 10 days of age, lipid synthesis occurs at almost the same intensity. At 30 days of age it increases significantly in the small intestinal mucosa and decreases in the large intestinal mucosa. Significant differences in the degree of use of [2-14C] acetate in the synthesis of individual lipid classes in the small intestinal mucosa of piglets at all stages of the study compared with the liver were revealed. The same features are observed for the synthesis of individual classes of lipids in the mucosa of the large intestine of piglets at 10 and 30 days of age. The obtained results indicate significant differences in lipid synthesis in the mucosa of the small and large intestine and in the liver of suckling piglets. Acetate is very intensively used for lipid synthesis during the first 30 days of piglets’ life. On the first day of life, lipogenesis is most active in the liver, but with age the lipid synthesis gradually activated in the intestinal mucosa.
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