M. Abdullah, B. Bela, A. Syam, M. Simadibrata, Sofy Meilany, Firda Annisa, Dian Amirulloh, D. Makmun, A. A. Rani
{"title":"基于磁性生物打印的印尼Cipto Mangunkusumo国立医院结直肠癌细胞原代3D细胞培养的建立","authors":"M. Abdullah, B. Bela, A. Syam, M. Simadibrata, Sofy Meilany, Firda Annisa, Dian Amirulloh, D. Makmun, A. A. Rani","doi":"10.1063/1.5125529","DOIUrl":null,"url":null,"abstract":"Colorectal cancer is the third most common malignancy with more than 1.2 million new annual cases in the world. Methods for propagating cancer cells range from conventional to the more advanced system. Cancer cells are routinely cultured in two-dimensional (2D) model. This method faces several limitations attributable to lack of structural architecture and nature condition. Three-dimensional (3D) model mimics in vivo environment more closely than the conventional 2D. This strategy may help in elucidating cancer biology and subsequently contribute to higher success of therapy. The goal of this study was to establish the primary 3D culture from colorectal cancer collected from patients in Cipto Mangunkusumo National Hospital. Cancer cells were extracted mechanically and grown in DMEM/F12 medium supplemented with serum and antibiotics. The NanoShuttle-PL was added to the cells and magnetic drive was used to form cell spheroids. There were 22 samples obtained with varying cancer stages viz. IIA, IIIB, IIIC, and IV. Primary culture of cancer cells successfully established by mechanically extracting the tumour tissues into small pieces. Results on magnetic bioprinting revealed that the 3D culture (spheroids) was successfully established rapidly. Further studies are needed to evaluate culture viability and cell compositions, as well as performing the chemosensitivity testing.","PeriodicalId":20581,"journal":{"name":"PROCEEDINGS OF THE 2ND INTERNATIONAL CONFERENCE ON BIOSCIENCES AND MEDICAL ENGINEERING (ICBME2019): Towards innovative research and cross-disciplinary collaborations","volume":"16 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2019-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"4","resultStr":"{\"title\":\"Establishment of primary 3D cell culture based on magnetic bioprinting for colorectal cancer cells from patients in Cipto Mangunkusumo National Hospital Indonesia\",\"authors\":\"M. Abdullah, B. Bela, A. Syam, M. Simadibrata, Sofy Meilany, Firda Annisa, Dian Amirulloh, D. Makmun, A. A. Rani\",\"doi\":\"10.1063/1.5125529\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Colorectal cancer is the third most common malignancy with more than 1.2 million new annual cases in the world. Methods for propagating cancer cells range from conventional to the more advanced system. Cancer cells are routinely cultured in two-dimensional (2D) model. This method faces several limitations attributable to lack of structural architecture and nature condition. Three-dimensional (3D) model mimics in vivo environment more closely than the conventional 2D. This strategy may help in elucidating cancer biology and subsequently contribute to higher success of therapy. The goal of this study was to establish the primary 3D culture from colorectal cancer collected from patients in Cipto Mangunkusumo National Hospital. Cancer cells were extracted mechanically and grown in DMEM/F12 medium supplemented with serum and antibiotics. The NanoShuttle-PL was added to the cells and magnetic drive was used to form cell spheroids. There were 22 samples obtained with varying cancer stages viz. IIA, IIIB, IIIC, and IV. Primary culture of cancer cells successfully established by mechanically extracting the tumour tissues into small pieces. Results on magnetic bioprinting revealed that the 3D culture (spheroids) was successfully established rapidly. 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Establishment of primary 3D cell culture based on magnetic bioprinting for colorectal cancer cells from patients in Cipto Mangunkusumo National Hospital Indonesia
Colorectal cancer is the third most common malignancy with more than 1.2 million new annual cases in the world. Methods for propagating cancer cells range from conventional to the more advanced system. Cancer cells are routinely cultured in two-dimensional (2D) model. This method faces several limitations attributable to lack of structural architecture and nature condition. Three-dimensional (3D) model mimics in vivo environment more closely than the conventional 2D. This strategy may help in elucidating cancer biology and subsequently contribute to higher success of therapy. The goal of this study was to establish the primary 3D culture from colorectal cancer collected from patients in Cipto Mangunkusumo National Hospital. Cancer cells were extracted mechanically and grown in DMEM/F12 medium supplemented with serum and antibiotics. The NanoShuttle-PL was added to the cells and magnetic drive was used to form cell spheroids. There were 22 samples obtained with varying cancer stages viz. IIA, IIIB, IIIC, and IV. Primary culture of cancer cells successfully established by mechanically extracting the tumour tissues into small pieces. Results on magnetic bioprinting revealed that the 3D culture (spheroids) was successfully established rapidly. Further studies are needed to evaluate culture viability and cell compositions, as well as performing the chemosensitivity testing.