甘蓝型油菜WRKY转录因子BnWRKY57的克隆及表达分析

Fatemeh Shirazi, H. Razi, A. Niazi, A. Alemzadeh
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引用次数: 7

摘要

WRKY转录因子在植物的多种生物过程中发挥着重要的调控作用,包括对非生物胁迫的响应。WRKY基因可能是转基因育种提高油菜抗逆性的潜在靶点。本研究旨在克隆和鉴定甘蓝型油菜WRKY57 (BnWRKY57)基因,分析2个不同耐旱性和耐盐性的甘蓝型油菜WRKY57基因在干旱和盐胁迫下的表达规律。对BnWRKY57基因全长882bp的编码区(GenBank登录号:MG699908)进行克隆和测序。BnWRKY57基因编码293个氨基酸的亲水性多肽。该基因与其他已知的芸苔科WRKY57s具有高度同源性。BnWRKY57基因启动子中含有参与植物激素、光、生物和非生物胁迫响应的顺式调控元件,提示该基因可能调控不同的信号通路。实时定量RT-PCR分析显示,BnWRKY57的表达对干旱和盐胁迫有响应。在胁迫条件下,BnWRKY57基因在甘蓝型油菜叶片和根系之间以及不同品种之间的表达模式存在差异。综上所述,BnWRKY57基因可能参与甘蓝型油菜对干旱和盐胁迫的适应性反应。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Molecular cloning and expression analysis of a stress-responsive WRKY transcription factor gene, BnWRKY57, from Brassica napus
WRKY transcription factors play important roles in regulation of various plant biological processes, including response to abiotic stresses. WRKY genes might be potential targets for transgenic breeding to enhance stress tolerance in rapeseed (Brassica napus). The present study aimed to clone and characterize WRKY57 (BnWRKY57) gene derived from B. napus and to analyze patterns of BnWRKY57 expression under drought and salt stresses in two B. napus cultivars with different levels of tolerance to drought and salt. The full-length coding region of BnWRKY57 gene with 882bp long (GenBank Accession Number: MG699908) was cloned and sequenced. BnWRKY57 gene encodes a hydrophilic polypeptide of 293 amino acids. It shared high homology with other known WRKY57s from Brassicaceae family. The promoter of BnWRKY57 gene contained cis regulatory elements involved in response to phytohormones, light, biotic and abiotic stresses suggesting this gene may play a role to modulate different signaling pathways. Real time quantitative RT-PCR analysis revealed that BnWRKY57 expression was responsive to drought and salt stresses. BnWRKY57 gene showed different expression patterns between leaves and roots and also between the B. napus cultivars under stress conditions. Overall, the findings suggest that BnWRKY57 gene may contribute to adaptive responses to drought and salt stresses in B. napus.
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