- Book学术

发布求助

文献互助智能选刊最新文献

Indonesian Journal of Pure and Applied Chemistry Pub Date : 2022-07-31 DOI:10.26418/indonesian.v5i2.56554

Syafri Syamsudin, A. Alimuddin, Berlian Sitorus

{"title":"ISOLASI DAN KARAKTERISASI SENYAWA FENOLIK DARI DAUN PUTAT (Planchonia valida Blume) (ISOLATION AND CHARACTERIZATION OF PHENOLIC COMPOUND FROM PUTAT LEAVES (Planchonia valida Blume))","authors":"Syafri Syamsudin, A. Alimuddin, Berlian Sitorus","doi":"10.26418/indonesian.v5i2.56554","DOIUrl":null,"url":null,"abstract":"Gallic acid have been isolated from the leaves of the Putat plant (Planchonia valida Blume). This study aimed to isolate and characterize isolates from Putat leaves by phytochemical and FTIR spectrophotometry. The research procedures included extraction by maceration, partitioning by fractionation, vacuum liquid chromatography (KCV), gravity column chromatography (KKG), thin layer chromatography (TLC), and FTIR analysis. A total of 1400 grams of putat leaf powder was macerated to produce 159.243 grams (11.38%) of methanol extract. A total of 101 grams of methanol extract was dechlorophyllated to obtain 21.430 grams (21.21%) of dechlorophyllated extract. The dechlorophyllated extract was partitioned gradually with n-hexane and ethyl acetate to produce n-hexane fractions of 0.341 grams (1.591%), and ethyl acetate of 10.143 grams (47.33%), and methanol of 9.170 grams (42.79%). A phytochemical test showed that in the dechlorophyllated extract, the methanol and ethyl acetate fractions contained alkaloids, phenolics, flavonoids, and terpenoids while the n-hexane fraction contained alkaloids and flavonoids. A total of 7 grams of ethyl acetate fraction was fractionated by vacuum liquid chromatography to produce fractions B1 to B8. The B4 fraction was continued to the separation step by gravity column chromatography so that the fractions S1 to S9 were obtained. The S4 fraction was continued to the separation stage using preparative thin layer chromatography (TLC) to produce isolates S4.1 to S4.5. Isolate S4.1 was tested for purity by two-dimensional TLC showed that the isolate was not pure. The results of the phytochemical test with 5% FeCl3 spray reagent indicated a positive phenolic compound which was strengthened by FTIR characterization of the presence of –OH, -CH aromatic, -CH aliphatic, C=C, C=O and C-O goups. Based on these results, it was concluded that the isolate from the dechlorophyllated extract of Putat leaves belonged to phenolic compounds.","PeriodicalId":13554,"journal":{"name":"Indonesian Journal of Pure and Applied Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2022-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Indonesian Journal of Pure and Applied Chemistry","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.26418/indonesian.v5i2.56554","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 0

摘要

没食子酸是从普氏植物(Planchonia valida Blume)的叶子中分离得到的。本研究旨在通过植物化学和FTIR分光光度法对普洱茶叶中分离物进行分离鉴定。研究方法包括浸提法、分馏法、真空液相色谱法、重力柱色谱法、薄层色谱法和红外光谱分析。共浸渍1400克putat叶粉，得到159.243克(11.38%)甲醇提取物。共对101克甲醇提取物进行脱叶绿素处理，得到21.430克(21.21%)的脱叶绿素提取物。将脱氯叶提取物与正己烷、乙酸乙酯逐步分割，得到正己烷组分0.341 g(1.591%)、乙酸乙酯组分10.143 g(47.33%)、甲醇组分9.170 g(42.79%)。植物化学试验表明，脱氯叶提取物中甲醇和乙酸乙酯组分含有生物碱、酚类物质、类黄酮和萜类物质，正己烷组分含有生物碱和类黄酮。用真空液相色谱法分离乙酸乙酯馏分共7 g，得到B1 ~ B8馏分。B4馏分继续进行重力柱色谱分离，得到S1 ~ S9馏分。用制备薄层色谱(TLC)将S4馏分继续分离得到分离物S4.1 ~ S4.5。对分离物S4.1进行了二维薄层色谱纯度检测，结果表明该分离物不纯。用5% FeCl3喷雾试剂进行植物化学试验，结果表明其为阳性酚类化合物，并通过FTIR表征得到-OH、-CH芳族、-CH脂肪族、C=C、C=O和C-O基团的存在。综上所述，从Putat叶片脱叶绿素提取物中分离得到的化合物属于酚类化合物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

ISOLASI DAN KARAKTERISASI SENYAWA FENOLIK DARI DAUN PUTAT (Planchonia valida Blume) (ISOLATION AND CHARACTERIZATION OF PHENOLIC COMPOUND FROM PUTAT LEAVES (Planchonia valida Blume))

Gallic acid have been isolated from the leaves of the Putat plant (Planchonia valida Blume). This study aimed to isolate and characterize isolates from Putat leaves by phytochemical and FTIR spectrophotometry. The research procedures included extraction by maceration, partitioning by fractionation, vacuum liquid chromatography (KCV), gravity column chromatography (KKG), thin layer chromatography (TLC), and FTIR analysis. A total of 1400 grams of putat leaf powder was macerated to produce 159.243 grams (11.38%) of methanol extract. A total of 101 grams of methanol extract was dechlorophyllated to obtain 21.430 grams (21.21%) of dechlorophyllated extract. The dechlorophyllated extract was partitioned gradually with n-hexane and ethyl acetate to produce n-hexane fractions of 0.341 grams (1.591%), and ethyl acetate of 10.143 grams (47.33%), and methanol of 9.170 grams (42.79%). A phytochemical test showed that in the dechlorophyllated extract, the methanol and ethyl acetate fractions contained alkaloids, phenolics, flavonoids, and terpenoids while the n-hexane fraction contained alkaloids and flavonoids. A total of 7 grams of ethyl acetate fraction was fractionated by vacuum liquid chromatography to produce fractions B1 to B8. The B4 fraction was continued to the separation step by gravity column chromatography so that the fractions S1 to S9 were obtained. The S4 fraction was continued to the separation stage using preparative thin layer chromatography (TLC) to produce isolates S4.1 to S4.5. Isolate S4.1 was tested for purity by two-dimensional TLC showed that the isolate was not pure. The results of the phytochemical test with 5% FeCl3 spray reagent indicated a positive phenolic compound which was strengthened by FTIR characterization of the presence of –OH, -CH aromatic, -CH aliphatic, C=C, C=O and C-O goups. Based on these results, it was concluded that the isolate from the dechlorophyllated extract of Putat leaves belonged to phenolic compounds.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Indonesian Journal of Pure and Applied Chemistry

自引率

0.00%

发文量