洋葱鳞茎腐病的发生、鉴定及初步生物防治

IF 0.6 4区 综合性期刊 Q3 MULTIDISCIPLINARY SCIENCES
K. Srimai, A. Akarapisan
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引用次数: 0

摘要

2022年,在泰国清迈Mae Wang区的低温仓库中观察到洋葱鳞茎腐烂。经形态学、生化鉴定、致病性检测和16S rRNA基因序列分析,分离得到的病原菌分别为Pantoea agglomerans和Burkholderia gladioli。生物防治被认为是一种比化学防治更安全、更可持续的防治策略,引起了人们对疾病防治的高度重视。从根际土壤中分离到芽孢杆菌BB22,并采用双重培养技术对其抑制洋葱鳞茎腐病病原菌生长的潜力进行了初步评价。结果表明,对团聚假单胞菌和剑兰假单胞菌的抑制区分别为30.1 mm和13.0 mm。利用16S rRNA和gyrA基因序列对Bacillus sp.分离物BB22进行了分子鉴定,确认其为velezensis芽孢杆菌。PCR扩增的bmyB、find、ituA和srfAA基因分别编码bacillomycin、fengycin D、iturin A和surfactin,显示了生产这些抗生素的潜力。本研究揭示了velezensis作为洋葱球茎腐烂致病菌生物防治剂的潜在应用前景。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Occurrence, Identification and Preliminary Biological Control of Bulb Rot of Onion (Allium cepa)
Onion bulb rot was observed in low-temperature warehouses in Mae Wang District, Chiang Mai, Thailand in 2022. The pathogens were isolated and identifi ed as Pantoea agglomerans and Burkholderia gladioli based on morphological and biochemical characterization, pathogenicity tests, and 16S rRNA gene sequences. Biological control is regarded as a safer and more sustainable strategy than chemical control and has elicited great attention for disease control. Bacillus sp. isolate BB22 was isolated from rhizosphere soil and was evaluated for its preliminary potential to inhibit the growth of onion bulb rot bacterial pathogens by the dual culture technique. The results showed inhibition zones of P. agglomerans of 30.1 mm and B. gladioli of 13.0 mm. Molecular identifi cation of Bacillus sp. isolate BB22 was performed by using 16S rRNA and gyrA gene sequences and confi rmed as Bacillus velezensis. PCR amplifi cation of the bmyB, fenD, ituA, and srfAA genes coding for bacillomycin, fengycin D, iturin A, and surfactin, respectively, showed the potential for the production of these antibiotics. This study revealed the potential application of B. velezensis as a biocontrol agent against bulb rot-causing bacterial pathogens of onions.
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来源期刊
Chiang Mai Journal of Science
Chiang Mai Journal of Science MULTIDISCIPLINARY SCIENCES-
CiteScore
1.00
自引率
25.00%
发文量
103
审稿时长
3 months
期刊介绍: The Chiang Mai Journal of Science is an international English language peer-reviewed journal which is published in open access electronic format 6 times a year in January, March, May, July, September and November by the Faculty of Science, Chiang Mai University. Manuscripts in most areas of science are welcomed except in areas such as agriculture, engineering and medical science which are outside the scope of the Journal. Currently, we focus on manuscripts in biology, chemistry, physics, materials science and environmental science. Papers in mathematics statistics and computer science are also included but should be of an applied nature rather than purely theoretical. Manuscripts describing experiments on humans or animals are required to provide proof that all experiments have been carried out according to the ethical regulations of the respective institutional and/or governmental authorities and this should be clearly stated in the manuscript itself. The Editor reserves the right to reject manuscripts that fail to do so.
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