电磁辐射和γ辐射诱导草种突变的研究

IF 0.8 4区生物学 Q3 AGRONOMY

Legume Research Pub Date : 2018-05-20 DOI:10.18805/LR-3981

Prabhat K. Singh, R. Sadhukhan

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引用次数: 5

摘要

本文研究了EMS和伽马射线单独和联合处理对三种不同基因型草种(Nirmal、Biol-212和Berhampur Local)的诱变诱导。联合治疗造成的生物损伤(致死性、损伤性和不育性)大于射线治疗和EMS治疗。Nirmal、Biol-212和Berhampur Local的LD50剂量分别为590 Gy、630 Gy和539 Gy。Nirmal和Biol-212的EMS超过0.5%，而Berhampur Local的EMS超过1%，被认为是致命的。3个品种的平均总突变率在γ辐射处理中最高(6.32%)，其次是EMS和联合处理(5.8%)。伽马射线和电磁辐射的最有效剂量/浓度分别为400戈瑞和0.5%电磁辐射。随着EMS浓度的增加，诱变效率增加，效果降低，但在伽马射线中，结果几乎呈反比关系。总的来说，无论基因型如何，伽马射线和EMS分别被发现是最有效和最有效的诱变剂。

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Ems and gamma radiation induced mutation in grasspea (Lathyrus sativus L.)

Present investigation has been undertaken to study the induction of mutation by individual and combination treatments of EMS and gamma rays in three diverged genotypes of grasspea viz. Nirmal, Biol-212 and Berhampur Local. Combination treatments caused the more biological damage (lethality, injury and sterility) followed by gamma rays and EMS alone. The LD50 dose of gamma rays for Nirmal, Biol-212 and Berhampur Local corresponded to 590 Gy, 630 Gy and 539 Gy respectively. Above 0.5% EMS for Nirmal and Biol-212 and above 1% EMS in case of Berhampur Local was found to be lethal. Average total mutation frequency of three varieties together was highest (6.32 %) in gamma radiation followed by both EMS and combination treatment (5.8%). The most efficient dose/concentration of gamma rays and EMS were found to be 400 Gy and 0.5% EMS respectively. Mutagenic efficiency increases and effectiveness decreases with increasing concentration of EMS but the consequence was showed almost inverse relationship in case of gamma rays. Overall, irrespective of genotypes, gamma rays and EMS respectively were found to be most efficient and effective mutagen.

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来源期刊

Legume Research AGRONOMY-

CiteScore

1.30

自引率

37.50%

发文量

256

审稿时长

6-12 weeks

期刊介绍： Legume Research An International Journal. The Journal is an official publication of Agricultural Research Communication Centre. It is designed to bring out the original research articles on genetics, breeding, physiology, bacterial avtivity, production, quality, biochemistry and seeds of legumes crops. The objective of the journal is to serve as a forum for scientific community to publish their research findings on legumes ans to provide basis for new research. Journal is being scanned in the important indexing and abstracting services.