干旱胁迫对菜豆生理特性及叶绿体分离的影响

IF 0.8 4区 生物学 Q3 AGRONOMY
Legume Research Pub Date : 2020-01-01 DOI:10.18805/LR-455
T. Zadražnik, J. Šuštar-Vozlič
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引用次数: 2

摘要

叶绿体参与许多细胞过程,在植物的逆境响应中起着重要作用。本研究报道了干旱对普通豆(Phaseolus vulgaris L.)不同生理特性的影响以及干旱胁迫和对照植株叶片叶绿体的分离。分析了两种对干旱反应不同的品种——台伯和更敏感的斯塔扎格斯基。相对含水量结果表明,不浇水6天后,植株处于轻度胁迫状态。干旱胁迫对两个品种的光合速率和叶绿素荧光参数均有不利影响。与干旱胁迫样品相比,两个品种对照样品的分离叶绿体产量至少高出三倍。检查分离的叶绿体的完整性,并用于蛋白质分离。研究结果表明,即使是轻微的干旱胁迫也会强烈影响完整叶绿体的分离。然而,完整的叶绿体被富集,适合下游蛋白质组学分析。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Impact of drought stress on physiological characteristics and isolation of chloroplasts in common bean (Phaseolus vulgaris L.)
Chloroplasts are involved in many cellular processes and play an important role in plant stress response. In this study, the effect of drought on different physiological characteristics and the isolation of chloroplasts from leaves of drought-stressed and control plants of common bean (Phaseolus vulgaris L.) are reported. Two cultivars differing in the response to drought were analysed-Tiber and more sensitive Starozagorski eern. The results of relative water content showed that plants were under mild stress conditions after six days without watering. Negative effects of drought stress on the photosynthetic rate and chlorophyll fluorescence parameters of both cultivars were observed. The yield of isolated chloroplasts in control samples of both cultivars was at least three times higher compared to drought-stressed samples. The intactness of the isolated chloroplasts was checked and used for protein isolation. The results of the study suggested that even mild drought stress strongly affected the isolation of intact chloroplasts. Still, intact chloroplasts were enriched and were suitable for downstream proteomic analysis.
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来源期刊
Legume Research
Legume Research AGRONOMY-
CiteScore
1.30
自引率
37.50%
发文量
256
审稿时长
6-12 weeks
期刊介绍: Legume Research An International Journal. The Journal is an official publication of Agricultural Research Communication Centre. It is designed to bring out the original research articles on genetics, breeding, physiology, bacterial avtivity, production, quality, biochemistry and seeds of legumes crops. The objective of the journal is to serve as a forum for scientific community to publish their research findings on legumes ans to provide basis for new research. Journal is being scanned in the important indexing and abstracting services.
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