蛋白质微阵列技术

生物技术通讯 Pub Date : 2003-12-11 DOI:10.1002/3527601554

Heng-jie Cao

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引用次数: 54

摘要

蛋白质芯片技术是随着DNA芯片技术的发展而发展起来的。最初为DNA微阵列开发的微射线表面、阵列和检测系统可以适用于蛋白质微阵列。蛋白质附着的不同表面已经被开发出来，以保持蛋白质在适当的位置和功能状态。基于荧光、化学发光、放射性的读出系统已被用于检测每个微点内的复杂结构。重组蛋白技术的发展提高了芯片检测的灵敏度。微阵列免疫分析具有可扩展、重复性好、样品少、可定量等特点，因此在生物医学研究中得到了广泛应用。本文综述了近年来蛋白质芯片的制备及其在免疫分析、医学诊断和蛋白质组学研究中的应用。

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Protein microarray technology

Protein microarray technology was developed with the development of DNA microarray technology.Microar-ray surfaces,arraying and detection systems that have initially been developed for DNA microarray can be adapted to protein microarray.The different surfaces for protein attachment have been developed in order to keep proteins in place and in a functional state.Readout systems based on fluorescence,chemiluminescence,radioactivity have been used to detect complex formation within each microspot.The development of recombinant proteins technology im-proved the sensitivity of detection on microarray.Immunoassays on microarray are scalable,reproducible,quantiliable with little sample and are consequently finding wide use in biomedical research.In this paper,some recent develop-ments in preparation of protein microarray and its application in immunoassays,medical diagnostis and proteome re-search are reviewed.

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来源期刊

生物技术通讯

自引率

0.00%

发文量

4941

期刊介绍： Letters in Biotechnology is an excellent journal officially approved by the General Administration of Press and Publication of the People's Republic of China for public publication. The journal was founded in 1989 and is sponsored by the Institute of Bioengineering of the Academy of Military Medical Sciences. It is a biology journal published publicly at home and abroad. The journal has columns such as research reports, technical methods, reviews, experience exchanges, special research bulletins, biological medicine gardens and knowledge introductions.