TAXONOMIC COMPOSITION, MICROSCOPIC INDICES OF URINARY TRACT MICROBIOTA IN MEN WITH CALCULUS PYELONEPHRITIS

The aim of the study – to investigate the taxonomic composition, qualitativemicroecological indicators (index of constancy, frequency of occurrence, indices of speciesrichness of Margalef, species diversity of Whittaker and species dominance of Simpsonand Berger-Parker) and the immunosuppressive effect of causative agents of calculouspyelonephritis on the activity of the complement system, lysozyme and inhibition of themain immunoglobulin classes (IgM, IgA).Material and methods. Microbiological (bacteriological and mycological) urineexamination was carried out in 30 men aged 37-65 years (48.81 ± 4.27 years), patientswith calculous pyelonephritis. The ecological method was used to reveal the mechanismsof colonization of the biotope by microbiota. The adhesive properties were studied onnative human erythrocytes of group I (0I). Anti-immunoglobulin activity was studied bythe method described in the work of I.S. Gaidash, etc.Results. Out of 30 samples of the research material, 34 strains of opportunistic gramnegative and gram-positive bacteria were isolated and identifed. The majority (86.67%)had a monoculture of enterobacteria, enterococci, associations consisting of two taxonswere found in 13.33% of patients. It has been shown that 97.06% of isolated and identifedstrains from the urinary tract exhibit adhesive activity of varying degrees of activity. Onestrain of S. saprophyticus also exhibits adhesive activity (the average adhesion index is1.83 ± 0.09), which is considered as a non-adhesive strain. All strains of enterobacteriaand gram-positive cocci that persist on the mucous membrane of the urinary tract ofmen with infectious and inflammatory processes of the urinary tract with calculouspyelonephritis inhibit (P <0.05) immunoglobulins of class A (IgA).Conclusions. The infectious and inflammatory process on the mucous membranes of theurinary tract in men with calculous pyelonephritis is caused by facultative anaerobicgram-negative enterobacteria and gram-positive cocci. The presence of an infectious andinflammatory process is confrmed by an acceleration of the erythrocyte sedimentationrate by 76.67% and other clinical and laboratory studies. Pathogenicity factors arerevealed in isolated and identifed opportunistic taxons, that determine the initial stagesof the infectious and inflammatory process (cytoadhesion and colonization of the urinarytract mucosa), inhibit the functional activity of class A and M of immunoglobulins.