在酿酒酵母中，Metacaspase缺失增加了与蛋白质合成和碳水化合物代谢相关的羰基化和酪氨酸磷酸化蛋白

Q4 Medicine

International Journal of Nutrition, Pharmacology, Neurological Diseases Pub Date : 2022-04-01 DOI:10.4103/ijnpnd.ijnpnd_18_21

M. Khan, Meghan Morgan, M. Essa, Mohammed Akbar, Gilles J. Guillemin, B. Song

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引用次数: 0

摘要

过氧化氢(H2O2)是一种氧化剂，可以诱导细胞中蛋白质的翻译后修饰(ptmp)。目前尚不清楚caspase抑制的白血病细胞或caspase缺失的酿酒酵母中是否积累了羰基化蛋白(CPs)。因此，我们旨在鉴定CPs并阐明metacaspase在调节PTMPs中的作用，并通过蛋白质组学方法鉴定/比较Δyca1突变体与野生型在H2O2暴露/不暴露下PTMPs的差异表达。我们发现，与H2O2相比，酵母中metacaspase基因(MCG)的缺失导致了大量与蛋白质合成和碳水化合物代谢相关的PTMPs的积累，这表明MCG参与了PTMPs的调控，并可以保护酵母免受氧化应激。

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Metacaspase Deletion Increases Carbonylated and Tyrosine-Phosphorylated Proteins associated with Protein Synthesis and Carbohydrate Metabolism in Saccharomyces cerevisiae

Hydrogen peroxide (H2O2) is an oxidant which could induce posttranslational modifications of proteins (PTMPs) in cells. It is still unknown that carbonylated proteins (CPs) were accumulated in caspase-suppressed leukemia cells or caspase-deleted Saccharomyces cerevisiae (yeast). Hence, we aimed to identify CPs and elucidate the role of metacaspase in regulating PTMPs and identify/compare the differentially expressed PTMPs in Δyca1 mutant compared to wild type with/without H2O2 exposure by proteomics approach. We found that deletion of the metacaspase gene (MCG) in yeast resulted in accumulation of high amounts of PTMPs associated with protein synthesis and carbohydrate metabolism compared to H2O2, which suggests that MCG is involved in the regulation of PTMPs and it could protect yeast from oxidative stress.

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来源期刊

International Journal of Nutrition, Pharmacology, Neurological Diseases Medicine-Pharmacology (medical)

CiteScore

0.80

自引率

0.00%

发文量

期刊介绍： The International Journal of Nutrition, Pharmacology, Neurological Diseases (IJNPND) is an international, open access, peer reviewed journal which covers all fields related to nutrition, pharmacology, neurological diseases. IJNPND was started by Dr. Mohamed Essa based on his personal interest in Science in 2009. This journal doesn’t link with any society or any association. The co-editor-in chiefs of IJNPND (Prof. Gilles J. Guillemin, Dr. Abdur Rahman and Prof. Ross grant) and editorial board members are well known figures in the fields of Nutrition, pharmacology, and neuroscience. First, the journal was started as two issues per year, then it was changed into 3 issues per year and since 2013, it publishes 4 issues per year till now. This shows the slow and steady growth of this journal. To support the reviewers and editorial board members, IJNPND offers awards to the people who does more reviews within one year. The International Journal of Nutrition, Pharmacology, Neurological Diseases (IJNPND) is published Quarterly. IJNPND has three main sections, such as nutrition, pharmacology, and neurological diseases. IJNPND publishes Research Papers, Review Articles, Commentaries, case reports, brief communications and Correspondence in all three sections. Reviews and Commentaries are normally commissioned by the journal, but consideration will be given to unsolicited contributions. International Journal of Nutrition, Pharmacology, Neurological Diseases is included in the UGC-India Approved list of journals.