水生氨基肽酶活性的小尺度异质性定量分析

IF 1.6 4区 环境科学与生态学 Q3 ECOLOGY
B. Gaas, J. Ammerman
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引用次数: 0

摘要

亮氨酸氨基肽酶(Leucine aminopeptidase, LAP)是参与肽水解的酶之一,有时被用来指示微生物中潜在的氮限制。小规模变异性有可能在时间或空间上混淆对LAP活动基本模式的解释。使用自动流动注射分析仪器来解决哈德逊河羽流(美国新泽西州)连续区域内LAP活动的小尺度变化。LAP活性变异系数(CV)约为0.5,偶有大于1.0的变异系数。其他生物参数(叶绿素荧光和硝酸盐浓度)的平均CVs相似,盐度的平均值要低得多。不同盐度下,LAP活性的变化平均为35 nmol l-1 h-1,跨区域的变化大于同一区域内的变化。在间隔<10 m的连续样品之间,LAP活性的差异为±100 nmol l-1 h-1。变异图分析表明,整个研究区存在52 nmol l-1 h-1的内在空间变异。LAP活性的大变化往往与盐度和叶绿素荧光的小变化相关,并且对采样频率敏感。这项研究的结论是,一个样本的LAP测量值实际上可以在零到平均水平的两倍之间,区域或时间之间的变化应该至少是2倍,这样才能在一定程度上确信活动的明显模式(或缺乏模式)是真实的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Quantification of small-scale heterogeneity in aquatic aminopeptidase activity
Leucine aminopeptidase (LAP) is one of the enzymes involved in the hydrolysis of peptides, and is sometimes used to indicate potential nitrogen limitation in microbes. Small-scale variability has the potential to confound interpretation of underlying patterns in LAP activity in time or space. An automated flow-injection analysis instrument was used to address the small-scale variability of LAP activity within contiguous regions of the Hudson River plume (New Jersey, USA). LAP activity had a coefficient of variation (CV) of ca. 0.5 with occasional values above 1.0. The mean CVs for other biological parameters—chlorophyll fluorescence and nitrate concentration—were similar, and were much lower for salinity. LAP activity changed by an average of 35 nmol l-1 h-1 at different salinities, and variations in LAP activity were higher crossing region boundaries than within a region. Differences in LAP activity were ±100 nmol l-1 h-1 between sequential samples spaced <10 m apart. Variogram analysis indicated an inherent spatial variability of 52 nmol l-1 h-1 throughout the study area. Large changes in LAP activity were often associated with small changes in salinity and chlorophyll fluorescence, and were sensitive to the sampling frequency. This study concludes that LAP measurements in a sample could realistically be expected to range from zero to twice the average, and changes between areas or times should be at least 2-fold to have some degree of confidence that apparent patterns (or lack thereof) in activity are real.
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来源期刊
Aquatic Microbial Ecology
Aquatic Microbial Ecology 环境科学-海洋与淡水生物学
CiteScore
3.30
自引率
0.00%
发文量
8
审稿时长
3.0 months
期刊介绍: AME is international and interdisciplinary. It presents rigorously refereed and carefully selected Research Articles, Reviews and Notes, as well as Comments/Reply Comments (for details see AME 27:209), Opinion Pieces (previously called ''As I See It'') and AME Specials. For details consult the Guidelines for Authors. Papers may be concerned with: Tolerances and responses of microorganisms to variations in abiotic and biotic components of their environment; microbial life under extreme environmental conditions (climate, temperature, pressure, osmolarity, redox, etc.). Role of aquatic microorganisms in the production, transformation and decomposition of organic matter; flow patterns of energy and matter as these pass through microorganisms; population dynamics; trophic interrelationships; modelling, both theoretical and via computer simulation, of individual microorganisms and microbial populations; biodiversity. Absorption and transformation of inorganic material; synthesis and transformation of organic material (autotrophic and heterotrophic); non-genetic and genetic adaptation; behaviour; molecular microbial ecology; symbioses.
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