活性炭电极伏安法同时测定扑热息痛和抗坏血酸:循环伏安法和方波伏安法的研究

IF 0.4 Q4 CHEMISTRY, MULTIDISCIPLINARY

Acta Chemica Iasi Pub Date : 2021-01-01 DOI:10.47743/achi-2021-2-0008

Yared Shewarega, Dereje Yenealem, Fikadu Siyum

{"title":"活性炭电极伏安法同时测定扑热息痛和抗坏血酸:循环伏安法和方波伏安法的研究","authors":"Yared Shewarega, Dereje Yenealem, Fikadu Siyum","doi":"10.47743/achi-2021-2-0008","DOIUrl":null,"url":null,"abstract":": Simultaneous voltammetric determination of paracetamol (PA) and ascorbic acid (AA) at activated glassy carbon electrode (aGCE) using cyclic voltammetry (CV) and square wave voltammetry (SWV) was studied. The electrochemical responses of PA and AA were compared at bare (bGCE) and aGCE. The aGCE displayed excellent electrochemical catalytic activities and decreased the over-potential for simultaneous determination of PA and AA. It was found that the redox process at aGCE for both PA and AA is adsorption-controlled process. The linear range, limit of quantification (LOQ) and detection limit (LOD) of PA were found to be 10 to 100 µM; 0.517 µM and 0.155 µM, respectively. Similarly, the linear range, LOQ and LOD of AA were found to be 0.4 to 0.95 mM, 6.32 µM and 1.89 µM, respectively. The validity of the proposed method was checked by using commercial drug which contain different amount of PA and AA and satisfactory percent recoveries were obtained.","PeriodicalId":6958,"journal":{"name":"Acta Chemica Iasi","volume":"45 1","pages":""},"PeriodicalIF":0.4000,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"SIMULTANEOUS VOLTAMMETRIC DETERMINATION OF PARACETAMOL AND ASCORBIC ACID USING ACTIVATED GLASSY CARBON ELECTRODE: CYCLIC VOLTAMMETRY AND SQUARE WAVE VOLTAMMETRY STUDY\",\"authors\":\"Yared Shewarega, Dereje Yenealem, Fikadu Siyum\",\"doi\":\"10.47743/achi-2021-2-0008\",\"DOIUrl\":null,\"url\":null,\"abstract\":\": Simultaneous voltammetric determination of paracetamol (PA) and ascorbic acid (AA) at activated glassy carbon electrode (aGCE) using cyclic voltammetry (CV) and square wave voltammetry (SWV) was studied. The electrochemical responses of PA and AA were compared at bare (bGCE) and aGCE. The aGCE displayed excellent electrochemical catalytic activities and decreased the over-potential for simultaneous determination of PA and AA. It was found that the redox process at aGCE for both PA and AA is adsorption-controlled process. The linear range, limit of quantification (LOQ) and detection limit (LOD) of PA were found to be 10 to 100 µM; 0.517 µM and 0.155 µM, respectively. Similarly, the linear range, LOQ and LOD of AA were found to be 0.4 to 0.95 mM, 6.32 µM and 1.89 µM, respectively. The validity of the proposed method was checked by using commercial drug which contain different amount of PA and AA and satisfactory percent recoveries were obtained.\",\"PeriodicalId\":6958,\"journal\":{\"name\":\"Acta Chemica Iasi\",\"volume\":\"45 1\",\"pages\":\"\"},\"PeriodicalIF\":0.4000,\"publicationDate\":\"2021-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Acta Chemica Iasi\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.47743/achi-2021-2-0008\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"CHEMISTRY, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta Chemica Iasi","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.47743/achi-2021-2-0008","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}

引用次数: 1

摘要

研究了循环伏安法(CV)和方波伏安法(SWV)在活性炭电极上同时测定扑热息痛(PA)和抗坏血酸(AA)的方法。比较了PA和AA在裸(bGCE)和aGCE下的电化学响应。aGCE表现出良好的电化学催化活性，降低了同时测定PA和AA的过电位。结果表明，PA和AA在aGCE上的氧化还原过程均为吸附控制过程。PA的线性范围、定量限(LOQ)和检出限(LOD)为10 ~ 100 μM;分别为0.517 μM和0.155 μM。同样，AA的线性范围为0.4 ~ 0.95 mM, LOQ为6.32 μM, LOD为1.89 μM。用不同PA和AA含量的市售药品对该方法进行了验证，得到了满意的回收率。关键词:抗坏血酸，对乙酰氨基酚，同步电化学测定，方波伏安法Yared Shewarega, e-mail: shewaregay@gmail.com扑热息痛(n -乙酰基-对氨基酚)是一种解热和轻微镇痛药物，实际上没有抗炎作用。在正常的治疗剂量下，它很容易被代谢并在尿液中完全消除，对健康没有任何极端影响。然而，在某些情况下过量服用会导致有毒代谢物的形成。抗坏血酸(AA)以其高抗氧化活性而闻名，临床上用于治疗和预防坏血病、普通感冒、精神疾病、艾滋病，并保护活细胞免受氧化损伤。它以其还原性(抗氧化活性)而闻名。因此，它代表了一个重要的质量指标，有助于食品的抗氧化性能。补充AA的使用加强了PA的主要有利作用，同时补偿了肝脏功能中的潜在毒性。另一方面，大多数文献报道了PA和AA之间的主要干扰，以及各种基质中的大量化学物质。以前的分析方法已经开发并常规用于同时测定PA和AA。然而，有些方法，如;色谱法、电泳法和分光光度法都有一些缺点，比如;分析时间长，需制样，程序复杂，投资成本高，性能不佳。电化学技术广泛应用于药物和生理液体中电活性化合物的测定。伏安法常用于药物的测定，以固体或碳电极材料为代表。它们具有优良的灵敏度、选择性、重复性和低的同时伏安法测定扑热息痛和抗坏血酸。109 .检测限，13 .温度范围宽，分析时间快，能测定药物的动力学和机理参数，既可用于鉴定又可用于定量。玻碳电极(GCE)具有较高的惰性、极小的孔隙尺寸、极小的渗透性、优异的生物相容性和极低的热膨胀系数。表面预处理改善了GCE表面的电化学性质。电化学预处理过程有助于获得准确、确定、可重现的电化学信号。通过在GCE表面引入不同的官能团，GCE的活化显著提高了电化学传感器对目标分析物的选择性和灵敏度。因此，本研究采用aGCE同时电化学测定PA和AA。研究了GCE活化前后PA和AA的循环伏安响应。与传统电极相比，aGCE具有更好的电化学性能。因此，aGCE被用于PA和AA的电化学研究。结果和讨论PA和AA的电化学行为可以看出(图1a)在-0.2 ~ 0.8 V的电位范围内没有观察到很好的氧化和还原峰。氧化峰电流宽，无还原峰，表明AA在裸GCE上的电化学反应是完全不可逆的，由于AA的氧化产物吸附在电极表面造成污染，没有得到可重复的电极响应。另一方面，在110 Yared Shewarega等人的实验中(图1b)表明，AA在aGCE上的氧化是不可逆的。此外，氧化峰向低正电位的移动表明，更快的电子转移反应需要更低的过电位。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

SIMULTANEOUS VOLTAMMETRIC DETERMINATION OF PARACETAMOL AND ASCORBIC ACID USING ACTIVATED GLASSY CARBON ELECTRODE: CYCLIC VOLTAMMETRY AND SQUARE WAVE VOLTAMMETRY STUDY

: Simultaneous voltammetric determination of paracetamol (PA) and ascorbic acid (AA) at activated glassy carbon electrode (aGCE) using cyclic voltammetry (CV) and square wave voltammetry (SWV) was studied. The electrochemical responses of PA and AA were compared at bare (bGCE) and aGCE. The aGCE displayed excellent electrochemical catalytic activities and decreased the over-potential for simultaneous determination of PA and AA. It was found that the redox process at aGCE for both PA and AA is adsorption-controlled process. The linear range, limit of quantification (LOQ) and detection limit (LOD) of PA were found to be 10 to 100 µM; 0.517 µM and 0.155 µM, respectively. Similarly, the linear range, LOQ and LOD of AA were found to be 0.4 to 0.95 mM, 6.32 µM and 1.89 µM, respectively. The validity of the proposed method was checked by using commercial drug which contain different amount of PA and AA and satisfactory percent recoveries were obtained.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Acta Chemica Iasi CHEMISTRY, MULTIDISCIPLINARY-

自引率

0.00%

发文量

审稿时长

12 weeks