Assay of Buspirone Hydrochloride in Tablets Using Kinetic Spectrophotometry

A simple kinetic method was developed for the determination of Buspirone hydrochloride (BUS) in pharmaceutical preparation. The method is based on kinetic investigation of the oxidation reaction of the drug with alkaline potassium permanganate at room temperature for a fixed time of 20 min. The absorbance of the green coloured manganate ion produced was measured at 610 nm. Alternatively, the decrease in the absorbance of permanganate ion after addition of the drug was measured at 525 nm. Calibration graphs in both procedures were linear over the concentration range 10-40 μg/mL. The different experimental parameters affecting the development and stability of the colours were carefully studied and optimized. The determination of BUS by rate constant method, fixed concentration method and fixed time method was also feasible with calibration equations obtained but the latter method was found to be more applicable.