Richard K. Wagner, Roger M. Hinson, Christina C. Apodaca, Nathan Hoeldtke, Trey Buchanan, Roderick F. Hume Jr., Byron C. Calhoun
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{"title":"脂多糖对灌注人胎盘子叶白细胞介素-6生成的影响","authors":"Richard K. Wagner, Roger M. Hinson, Christina C. Apodaca, Nathan Hoeldtke, Trey Buchanan, Roderick F. Hume Jr., Byron C. Calhoun","doi":"10.1002/1520-6661(200011/12)9:6<351::AID-MFM1006>3.0.CO;2-5","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n \n <section>\n \n <h3> Objective</h3>\n \n <p>To determine if lipopolysaccharide (LPS) alters production of interleukin-6 (IL-6) or vascular tone in perfused placental cotyledons.</p>\n </section>\n \n <section>\n \n <h3> Methods</h3>\n \n <p>Control and study cotyledons from nine placentas were perfused for 3 h. Study cotyledons received LPS in concentrations of 0.01 mcg/ml (n = 3), 0.1 mcg/ml (n = 3), or 1.0 mcg/ml (n = 3). Effluents were collected at 30, 60, 120, and 180 min following infusion with LPS. IL-6 concentrations were measured by enzyme-linked immunosorbant assay. Perfusion pressures were recorded at 10-min intervals. Data were analyzed using ANOVA for repeated measures.</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p>IL-6 production significantly increased over time in both the study and control cotyledons (<i>P</i> = 0.002). LPS treatment did not affect IL-6 production (<i>P</i> = 0.85) and there were no observable dose effects (<i>P</i> = 0.13). Perfusion pressures did not differ (<i>P</i> = 0.16).</p>\n </section>\n \n <section>\n \n <h3> Conclusions</h3>\n \n <p>The isolated perfused placental cotyledon produces IL-6 and concentrations increase over time. LPS does not alter production of IL-6 or fetoplacental vascular tone. J. Matern.-Fetal Med. 2000;9:351–355. © 2000 Wiley-Liss, Inc.</p>\n </section>\n </div>","PeriodicalId":79464,"journal":{"name":"The Journal of maternal-fetal medicine","volume":"9 6","pages":"351-355"},"PeriodicalIF":0.0000,"publicationDate":"2001-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/1520-6661(200011/12)9:6<351::AID-MFM1006>3.0.CO;2-5","citationCount":"2","resultStr":"{\"title\":\"Effects of lipopolysaccharide on interleukin-6 production in perfused human placental cotyledons†\",\"authors\":\"Richard K. Wagner, Roger M. Hinson, Christina C. Apodaca, Nathan Hoeldtke, Trey Buchanan, Roderick F. Hume Jr., Byron C. Calhoun\",\"doi\":\"10.1002/1520-6661(200011/12)9:6<351::AID-MFM1006>3.0.CO;2-5\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n \\n \\n <section>\\n \\n <h3> Objective</h3>\\n \\n <p>To determine if lipopolysaccharide (LPS) alters production of interleukin-6 (IL-6) or vascular tone in perfused placental cotyledons.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Methods</h3>\\n \\n <p>Control and study cotyledons from nine placentas were perfused for 3 h. Study cotyledons received LPS in concentrations of 0.01 mcg/ml (n = 3), 0.1 mcg/ml (n = 3), or 1.0 mcg/ml (n = 3). Effluents were collected at 30, 60, 120, and 180 min following infusion with LPS. IL-6 concentrations were measured by enzyme-linked immunosorbant assay. Perfusion pressures were recorded at 10-min intervals. Data were analyzed using ANOVA for repeated measures.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Results</h3>\\n \\n <p>IL-6 production significantly increased over time in both the study and control cotyledons (<i>P</i> = 0.002). LPS treatment did not affect IL-6 production (<i>P</i> = 0.85) and there were no observable dose effects (<i>P</i> = 0.13). Perfusion pressures did not differ (<i>P</i> = 0.16).</p>\\n </section>\\n \\n <section>\\n \\n <h3> Conclusions</h3>\\n \\n <p>The isolated perfused placental cotyledon produces IL-6 and concentrations increase over time. LPS does not alter production of IL-6 or fetoplacental vascular tone. J. Matern.-Fetal Med. 2000;9:351–355. © 2000 Wiley-Liss, Inc.</p>\\n </section>\\n </div>\",\"PeriodicalId\":79464,\"journal\":{\"name\":\"The Journal of maternal-fetal medicine\",\"volume\":\"9 6\",\"pages\":\"351-355\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2001-02-15\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1002/1520-6661(200011/12)9:6<351::AID-MFM1006>3.0.CO;2-5\",\"citationCount\":\"2\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"The Journal of maternal-fetal medicine\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1002/1520-6661%28200011/12%299%3A6%3C351%3A%3AAID-MFM1006%3E3.0.CO%3B2-5\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"The Journal of maternal-fetal medicine","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/1520-6661%28200011/12%299%3A6%3C351%3A%3AAID-MFM1006%3E3.0.CO%3B2-5","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
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