{"title":"一种新的流式细胞术方案应用于curares麻醉期事故的诊断和预防的兴趣","authors":"J. Sainte-Laudy , I. Orsel","doi":"10.1016/j.allerg.2008.06.008","DOIUrl":null,"url":null,"abstract":"<div><h3>Aim of the study</h3><p>We tested here a new cytometric method based on the use of two activation markers (CD63 and IgE) and result's expression in activation index (AI).</p></div><div><h3>Material and methods</h3><p>Twenty-six allergic patients and 23 controls having received a neuromuscular blocker (NMB) have been selected on the basis of clinical history, skin tests and specific IgEs. NMB were tested mixed (<em>n</em> <!-->=<!--> <!-->26) or alone (injectable form, (<em>n</em> <!-->=<!--> <!-->30) and allergens commercialized for flow cytometry (<em>n</em> <!-->=<!--> <!-->9)). Concentrations used were the same than for IDR according to the recommandations of the French Society of Anaesthesia and Resuscitation (SFAR). The cytometric protocol used was the IgE/CD63 protocol, results being expressed in percentage CD63 and in AI calculated by a specific algorithm involving the mean fluorescence intensity (MFI) of the IgE+ and the CD63+ and − populations.</p></div><div><h3>Results</h3><p>In the technical described here NMB exhibited no toxicity even for concentretions 10 times higher than concentrations used for diagnosis. With the injectable form, the respective sensitivities observed for CD63 alone and the AI were 68 and 100% for the mixture of NMB and, 67 and 100% for the isolated NMB. With the NMB commercialized for flow cytometry, sensitivities were 47 and 100%. Specificities were 100% for CD63 alone and 98% for the AI. The link (Chi<sup>2</sup>) between skin tests and flow cytometry was significant for the two markers and the two allergen types. Correlation between AI and specific IgEs was not significant.</p></div><div><h3>Conclusion</h3><p>The new flow cytometric protocol described here has the necessary characteristics of a biological test which could be included in the pre-anaesthetic check up.</p></div>","PeriodicalId":92953,"journal":{"name":"Revue francaise d'allergologie et d'immunologie clinique","volume":"48 7","pages":"Pages 470-475"},"PeriodicalIF":0.0000,"publicationDate":"2008-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.allerg.2008.06.008","citationCount":"3","resultStr":"{\"title\":\"Intérêt d’un nouveau protocole de cytomètrie en flux appliqué au diagnostic et à la prévention des accidents peranesthésiques aux curares\",\"authors\":\"J. Sainte-Laudy , I. Orsel\",\"doi\":\"10.1016/j.allerg.2008.06.008\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Aim of the study</h3><p>We tested here a new cytometric method based on the use of two activation markers (CD63 and IgE) and result's expression in activation index (AI).</p></div><div><h3>Material and methods</h3><p>Twenty-six allergic patients and 23 controls having received a neuromuscular blocker (NMB) have been selected on the basis of clinical history, skin tests and specific IgEs. NMB were tested mixed (<em>n</em> <!-->=<!--> <!-->26) or alone (injectable form, (<em>n</em> <!-->=<!--> <!-->30) and allergens commercialized for flow cytometry (<em>n</em> <!-->=<!--> <!-->9)). Concentrations used were the same than for IDR according to the recommandations of the French Society of Anaesthesia and Resuscitation (SFAR). The cytometric protocol used was the IgE/CD63 protocol, results being expressed in percentage CD63 and in AI calculated by a specific algorithm involving the mean fluorescence intensity (MFI) of the IgE+ and the CD63+ and − populations.</p></div><div><h3>Results</h3><p>In the technical described here NMB exhibited no toxicity even for concentretions 10 times higher than concentrations used for diagnosis. With the injectable form, the respective sensitivities observed for CD63 alone and the AI were 68 and 100% for the mixture of NMB and, 67 and 100% for the isolated NMB. With the NMB commercialized for flow cytometry, sensitivities were 47 and 100%. Specificities were 100% for CD63 alone and 98% for the AI. The link (Chi<sup>2</sup>) between skin tests and flow cytometry was significant for the two markers and the two allergen types. Correlation between AI and specific IgEs was not significant.</p></div><div><h3>Conclusion</h3><p>The new flow cytometric protocol described here has the necessary characteristics of a biological test which could be included in the pre-anaesthetic check up.</p></div>\",\"PeriodicalId\":92953,\"journal\":{\"name\":\"Revue francaise d'allergologie et d'immunologie clinique\",\"volume\":\"48 7\",\"pages\":\"Pages 470-475\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2008-11-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/j.allerg.2008.06.008\",\"citationCount\":\"3\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Revue francaise d'allergologie et d'immunologie clinique\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0335745708001780\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Revue francaise d'allergologie et d'immunologie clinique","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0335745708001780","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Intérêt d’un nouveau protocole de cytomètrie en flux appliqué au diagnostic et à la prévention des accidents peranesthésiques aux curares
Aim of the study
We tested here a new cytometric method based on the use of two activation markers (CD63 and IgE) and result's expression in activation index (AI).
Material and methods
Twenty-six allergic patients and 23 controls having received a neuromuscular blocker (NMB) have been selected on the basis of clinical history, skin tests and specific IgEs. NMB were tested mixed (n = 26) or alone (injectable form, (n = 30) and allergens commercialized for flow cytometry (n = 9)). Concentrations used were the same than for IDR according to the recommandations of the French Society of Anaesthesia and Resuscitation (SFAR). The cytometric protocol used was the IgE/CD63 protocol, results being expressed in percentage CD63 and in AI calculated by a specific algorithm involving the mean fluorescence intensity (MFI) of the IgE+ and the CD63+ and − populations.
Results
In the technical described here NMB exhibited no toxicity even for concentretions 10 times higher than concentrations used for diagnosis. With the injectable form, the respective sensitivities observed for CD63 alone and the AI were 68 and 100% for the mixture of NMB and, 67 and 100% for the isolated NMB. With the NMB commercialized for flow cytometry, sensitivities were 47 and 100%. Specificities were 100% for CD63 alone and 98% for the AI. The link (Chi2) between skin tests and flow cytometry was significant for the two markers and the two allergen types. Correlation between AI and specific IgEs was not significant.
Conclusion
The new flow cytometric protocol described here has the necessary characteristics of a biological test which could be included in the pre-anaesthetic check up.
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