提取工艺对商品杏仁水提物营养成分及抗氧化、抗菌活性的影响

E. Horozić, J. Suljagić, Jasna R Gojkovic, Eldin Halilčević, Darja Kubiček, Ermina Cilović Kazarević
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引用次数: 1

摘要

本文研究了不同提取工艺对商品杏核水提物的植物化学成分和生物活性的影响。提取方法有浸渍法、超声波法和索氏法。对提取物中总酚、总黄酮、生物元素含量、抗氧化活性和抗菌活性进行了分析。采用DPPH和FRAP法分析其体外抗氧化活性。采用扩散技术对ATCC收集的参比菌株进行抑菌筛选。超声波浸渍法提取的黄酮类化合物和总酚含量最高。这些技术已被证明是提取宏量和微量营养素的最佳方法。在300 rpm下浸泡3小时得到的水提物与其他技术得到的水提物相比,对被测微生物有更大的抑制作用。索氏提取法是提取杏核活性成分效率最低的方法,干浸出率最低,仅为5.5%。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Influence of extraction technique on nutrient content, antioxidant and antimicrobial activity of aqueous extracts of commercial apricot kernels
This paper presents the results of influence of extraction technique on phytochemical composition and biological activity of aqueous extracts of commercial apricot kernels. Three techniques were used for extraction: maceration, ultrasonic and Soxhlet extraction. The content of total phenols, flavonoids, bioelements, antioxidant and antimicrobial activity was analyzed in the extracts. Antioxidant activity was analyzed in vitro using DPPH and FRAP methods. Antimicrobial screening was performed by diffusion technique on reference strains from the ATCC collection. The content of total phenols and flavonoids is highest in extracts obtained by ultrasonic extraction and maceration. These techniques have proven to be the best for the extraction of macro and micronutrients. The aqueous extract obtained by maceration at 300 rpm for 3 hours showed a greater inhibitory effect against the tested microorganisms, compared to the extracts obtained by other techniques. The least efficient method of extracting bioactive components from apricot kernels is Soxhlet extraction, with the lowest dry extract yield of 5.5%.   
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