金属-有机沸石框架脲酶固定化的高灵敏度SPR尿素生物传感器

2021 19th International Conference on Optical Communications and Networks (ICOCN) Pub Date : 2021-08-23 DOI:10.1109/ICOCN53177.2021.9563719

Liangliang Cheng, Wanlu Zheng, Like Li, Ya‐nan Zhang

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引用次数: 1

摘要

提出了一种高灵敏度的多模薄核多模表面等离子体共振(SPR)结构，并将其应用于尿素传感中。在薄芯纤维上溅射金膜激发SPR，然后在探针上包裹脲酶包埋在沸石咪唑酸酯框架(ZIF -8/脲酶)形成的酶包衣，实现高度特异的尿素生物传感器。尿素和脲酶在沸石咪唑酸骨架中的结合会引起SPR探针折射率的变化，从而引起SPR共振光谱的波长偏移。实验结果表明，共振波长与尿素浓度在1 ~ 10 mM范围内具有良好的线性关系，灵敏度可达4 nm/mM。

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Highly-Sensitive SPR Urea Biosensor Based on Urease Immobilized in Metal-Organic Zeolite Framework

A highly-sensitive multi-mode thin-core multi-mode (MTM) surface plasmon resonance (SPR) structure is proposed and applied in urea sensing. The thin-core fiber is sputtered with gold film to excite SPR, and then the probe is coated with enzyme coating that is formed by embedding urease in zeolitic imidazolate framework (ZIF -8/Urease) to realize a highly specific urea biosensor. The bind of urea and urease in the zeolitic imidazolate framework will cause the change of refractive index of the SPR probe, which will then induce the wavelength shift of SPR resonance spectrum. Experimental results demonstrated that the resonance wavelength has a good linear relationship with the urea concentration in the range of 1–10 mM, and the sensitivity can reach to 4 nm/mM.

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2021 19th International Conference on Optical Communications and Networks (ICOCN)

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