The Feed of Local Beef Bone Marrow Substitution during Pregnancy affects the Increase in Growth Hormone Offsprings Production of Thirty and Sixty Days Old Mice

Growth hormone (GH) deficiency during the growth phase will cause the disruption, especially in phases of infant, childhood, and puberty. Local beef bone marrow in Central Sulawesi of Indonesia contains fatty and amino acids, which needed for supporting the proliferation of somatotroph cells of fetal pituitary. This study aims to evaluate the potential of marrow-substituted feed during pregnancy against somatotroph-pituitary cell proliferation seen from the potential of the mice gland that produces the GH. The design of the research uses a complete randomized design (RAL) with a single factor. Fifty female rats and twenty male rats were adapted for two weeks after the estrus was mated in one cage. After the pregnancy, the mother rat was separated into another cage, and then it was intervened with the feed of bone marrow substitution that had been prepared. Then at thirty and sixty days of age, the offsprings were anesthetized with ketamine-xylazine, and the blood was taken from the heart. The GH serum was determined by the ELISA method. The results showed that there were significant differences in the concentration of GH rat-offspring from the mothers fed by the normal feed, IUGR feed, Donggala cattle’s bone marrow-substituted feed, and Bali cattle’s bone marrow-substituted feed, at thirty and sixty days of age (α<0.05).  Conclusion: The local cattle’s marrow from the Donggala or Bali, which is substituted onto the feed then is intervened during the pregnancy can increase the growth hormone production of the offspring born at he age of thirty and sixty days.