紫Physalis peruviana L. (Solanaceae)水提液和甲醇提液的抑菌活性、细胞毒性和定性植物化学成分

S. Njoroge, J. Mbaria, G. Aboge, G. Moriasi
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引用次数: 0

摘要

背景:与微生物感染相关的高发病率和死亡率以及由于使用不当和耐药性而导致的常规抗生素无效需要替代策略。目的:根据药用资料,对药用植物紫Physalis peruviana L. (Solanaceae)水提液和甲醇提液的抑菌活性、细胞毒性和植物化学成分进行定性研究。方法:采用圆盘扩散法对其抑菌活性进行研究。采用卤虾致死性实验法测定研究提取物对卤虾的细胞毒性。采用标准程序对研究提取物进行定性植物化学分析。结果:两种提取物在浓度≤50µg/ml时对铜绿假单胞菌均无抑菌活性,对其他微生物均无抑菌活性或略有抑菌活性。值得注意的是,水提物对大肠杆菌和金黄色葡萄球菌具有高至极高的活性,对蜡样芽孢杆菌具有极高至显著的活性。甲醇提取物在浓度≥200µg/ml时对大肠杆菌、金黄色葡萄球菌、蜡样芽孢杆菌和白色念珠菌均有显著的抑制作用。水提物的最低抑菌浓度(mic)分别为50 μg/ml(大肠杆菌和蜡样芽孢杆菌)、100 μg/ml(金黄色葡萄球菌)和200 μg/ml(白色念珠菌)。水提物对大肠杆菌和蜡样芽孢杆菌的最低杀菌浓度为100 μg/ml,对金黄色葡萄球菌的最低杀菌浓度为200 μg/ml,对白色念珠菌的最低杀菌浓度为400 μg/ml。甲醇提取物对大肠杆菌、金黄色葡萄球菌和蜡样芽孢杆菌的mic为25 μg/ml,对白色念珠菌的mic为100 μg/ml,对大肠杆菌、金黄色葡萄球菌和蜡样芽孢杆菌的mic为100 μg/ml,对白色念珠菌的MFC为200 μg/ml。水溶液无毒,甲醇提取物对盐水对虾有轻微毒性,说明其相对安全。在研究提取物中检测到各种植物化学物质,描绘了广泛的药理活性,包括抗菌活性。结论:紫杉叶水提液和醇提液可作为一种安全有效的新型抗菌药物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Antimicrobial activity, cytotoxicity, and qualitative phytochemical composition of aqueous and methanolic leaf extracts of Physalis peruviana L. (Solanaceae)
Background: The high morbidity and mortality associated with microbial infections and the ineffectiveness of conventional antibiotics due to inappropriate use and resistance warrant alternative stratagems. Aim: We investigated the antimicrobial, cytotoxicity, and qualitative phytochemical composition of the aqueous and methanolic leaf extracts of Physalis peruviana L. (Solanaceae) based on its ethnomedicinal information. Methods: The antimicrobial activity of the extracts was investigated using the disk diffusion technique. The brine shrimp lethality assay method was used to determine the cytotoxicity of the study extracts on brine shrimp nauplii. Qualitative phytochemistry of the study extracts was performed using standard procedures. Results: The two extracts did not possess antimicrobial activity against P. aeruginosa and were either inactive or slightly inactive against the other microbes at concentrations of ≤ 50 µg/ml. Notably, the aqueous extract exhibited high to very high activities against E. coli and S. aureus and very high to remarkable activities against B. cereus. The methanolic extract showed remarkable activity at concentrations of ≥200 µg/ml against E. coli, moderate to very high against S. aureus, high to very high against B. cereus, and moderate to high against C. albicans. The aqueous extract's minimum inhibitory concentrations (MICs) were 50 μg/ml (E. coli and B. cereus), 100 μg/ml (S. aureus), and 200 μg/ml (C. albicans). The minimum bactericidal concentration concentrations (MBCs) of the aqueous extract were 100 μg/ml (E. coli and B. cereus), 200 μg/ml (S. aureus), while the minimum fungicidal concentration (MFC) for C. albicans was 400 μg/ml. The MICs of the methanolic extract were 25 μg/ml for E. coli, S. aureus, and B. cereus and 100 μg/ml for C. albicans, and the MBCs were 100 μg/ml for E. coli, S. aureus, and B. cereus, and the MFC for C. albicans was 200 μg/ml. The aqueous was non-toxic, while the methanolic extract was slightly toxic to brine shrimp nauplii, indicating their relative safety. Various phytochemicals were detected in the study extracts, depicting broad pharmacologic activity, including antimicrobial activity. Conclusion: The aqueous and methanolic leaf extracts of P. peruviana may serve as a source of efficacious and safe novel antimicrobial agents upon further evaluation.
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