尼日利亚埃努古大都市Ogbete Main市场和Oye Emene市场零售的麻辣烤肉(Suya)的微生物质量

Amadi E.C., Nwangwu C.C, Yusuf H.I.
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摘要

对尼日利亚埃努古Ogbete主要市场(地点1)和Oye Emene市场(地点2)零售的“Suya”即食辛辣肉制品的微生物质量进行了评估,以大肠杆菌污染为目标。48份牛肉、肝脏、肠道和鸡肉的“suya”样品均质,用无菌蒸馏水连续稀释,并使用倒盘技术镀入伊红亚甲基蓝琼脂中。根据培养特性、革兰氏染色反应和生化(IMViC)试验对分离菌株进行鉴定,并采用16S rRNA基因进行分子鉴定。结果表明,所有样品均分离到大肠杆菌。在接种时,所有大肠杆菌分离株的总菌落计数(TCC)远高于已知的肠致病性菌株的感染剂量。地点1/2 2015年4 - 8月检测样品TCC最小值分别为:5.6 × 10/6.0 × 10;和4.4 ×10/3.2 x 10,分别在2016年9月和11月的Locations1/2测试中。TCC最高的4种为牛肠素。综上所述,出售给公众的“苏亚”都是被污染的,可能是粪便来源;因此不适合人类食用。该研究还强调需要改进suya产品的监测系统,以加强良好的食品卫生规范。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Microbial Quality of Spicy Roasted Meat (Suya) Retailed in Ogbete Main Market and Oye Emene Market, in Enugu Metropolis, Nigeria
Microbiological quality, targeting contamination with Escherichia coli, of ready-to-eat spicy meat product, “Suya” retailed in Ogbete Main Market (Location 1) and Oye Emene Market (Location 2), in Enugu, Nigeria was evaluated. Forty-eight samples of the “suya,” in forms of beef, liver, intestine, and chicken, were homogenized and serially diluted with sterile distilled water and plated into Eosin Methylene Blue agar using Pour Plate Technique. Identification of isolates were based on cultural characteristics, Gram stain reaction and Biochemical (IMViC) test, and confirmed by molecular test using 16S rRNA gene. Result showed that E coli were isolated from all the samples. Total colony counts (TCC) of all E.coli isolates were at inoculums much greater than the known infective dose for the Enteropathogenic strains. Least TCC in the April-August 2015 test samples from Locations 1/2 were: 5.6 x10/6.0 x 10, respectively; and 4.4 ×10/3.2 x 10, respectively in Locations1/2 in the SeptemberNovember 2016 test. The four highest TCC were from the cow intestine suya. In conclusion, “suya” sold to the public are contaminated, probably of faecal origin; therefore not fit for human consumption. This study also underscores the need for improved surveillance system on suya products, to enforce good food hygiene practices.
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