日本猪粪便、感染肠和农场环境中猪毛线虫和卵的分子鉴定

IF 0.5 4区农林科学 Q4 AGRICULTURE, MULTIDISCIPLINARY

Jarq-japan Agricultural Research Quarterly Pub Date : 2020-07-01 DOI:10.6090/JARQ.54.271

Ryunosuke Muramatsu, R. Sato, Narihisa Onuma, K. Sasai, T. Shibahara, M. Matsubayashi

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引用次数: 1

摘要

猪毛虫是猪体内常见的一种寄生虫，根据最近开发的分子方法的研究，它被认为会感染人类。然而，这种分子检测方法的实用性尚未得到检验。在本研究中，我们评估了基于pcr和测序的分子方法检测猪绦虫的几个阶段。针对线粒体大核糖体亚基的PCR成功扩增了样品中的DNA。分离株序列与来自中国和厄瓜多尔的分离株形成系统发育集群。从少量虫卵(约20个虫卵)和经组织病理学分析的虫卵片段中提取的dna是有效的。这是日本首次报道猪链球菌的分子鉴定。附加关键词:分子检测，线虫，寄生虫，PCR

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Molecular Identification of Trichuris suis Worms and Eggs in Pig Feces, Infected Intestines, and Farm Environments in Japan

Trichuris suis is a common parasite in pigs that is thought to infect humans based on studies using recently developed molecular methods. However, the utility of such molecular methods for detection has not been examined. In the present study, we assessed PCRand sequencing-based molecular methods for the detection of several stages of T. suis. PCR targeting the mitochondrial large ribosomal subunit was successful with amplification of DNA from the samples. The sequences of the isolates formed a phylogenetic cluster with isolates from China and Ecuador. The assay was effective by using DNAs extracted from a low number of eggs (> 20 eggs) and the fragments of worms after histopathologic analyses. This is the first report describing the molecular identification of T. suis from pigs in Japan. Discipline: Animal Science Additional key words: molecular detection, nematode, parasite, PCR

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来源期刊

Jarq-japan Agricultural Research Quarterly 农林科学-农业综合

CiteScore

1.00

自引率

25.00%

发文量

审稿时长

>36 weeks

期刊介绍： The Japan Agricultural Research Quarterly (JARQ) is a publication of the Japan International Research Center for Agricultural Sciences (JIRCAS), which provides readers overseas with the latest information on key achievements and developments in agricultural research in Japan, with the expectation that this information would contribute to the agricultural development of countries in tropical and subtropical regions.