具有28个残基电荷周期的蛋白质的核定位

IF 0.4 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
N. Sakiyama, R. Ke, R. Sawada, M. Sonoyama, S. Mitaku
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引用次数: 5

摘要

最近在人类蛋白质组中发现了具有28个残基电荷周期性的蛋白质(PCP28),许多带注释的PCP28位于细胞核中(Ke et al., Jpn.)。j:。物理,2007)。通过分析氨基酸序列的物理性质,检测核PCP28和细胞质PCP28的物理化学差异,并开发软件系统对两类PCP28进行分类。在细胞核和细胞质PCP28中,整个序列的全局参数和正电荷密度最高的片段附近的局部参数存在显著差异。整体分类分数包括脯氨酸和半胱氨酸的密度以及负电荷密度,局部分类分数包括电荷分布的对称性、半胱氨酸的密度和正电荷密度。使用全局和局部评分建立了预测系统,其灵敏度和特异性分别为92%和88%。利用与预测系统相关的参数,讨论了蛋白质向细胞核易位的机制。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Nuclear localization of proteins with a charge periodicity of 28 residues
Proteins with a charge periodicity of 28 residues (PCP28) were found recently in the human proteome, and many of the annotated PCP28 were located in the nucleus (Ke et al., Jpn. J. Appl. Phys. 2007). The physical properties of the amino acid sequences were analyzed to detect the difference in the physicochemistry between the nuclear and cytoplasmic PCP28 and develop a software system to classify the two types of PCP28. A significant difference in the global parameters from the entire sequence and the local parameters around a segment with the highest positive charge density was found between the nuclear and cytoplasmic PCP28. The global classification score included the densities of proline and cysteine, and the negative charge density, while the local score included the symmetry of the charge distribution, the density of cysteine, and the positive charge density. A prediction system was developed using the global and local scores, which possessed a sensitivity and specificity of 92% and 88%, respectively. The mechanism of translocation of proteins to the nucleus is discussed using the parameters relevant to the predictive system.
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来源期刊
Chem-Bio Informatics Journal
Chem-Bio Informatics Journal BIOCHEMISTRY & MOLECULAR BIOLOGY-
CiteScore
0.60
自引率
0.00%
发文量
8
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