UFLC-PDA对红枣果皮、果肉和种子酚类物质的表征及抗氧化活性测定

Aysun Yücetepe, A. Duran, B. Özçelik
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摘要

本研究的目的是表征红枣的果皮、果肉和种子组织,以确定总酚含量、花青素含量、抗氧化活性和酚类特征。用两种不同的方法测定了样品的抗氧化电位-2-二苯基-2-吡啶肼基(DPPH)自由基清除活性和铜还原能力(CUPRAC)。采用pH差法测定样品中单体花青素的含量。采用Folin Ciocalteu法测定提取物中总酚含量。此外,采用光电二极管阵列检测器(PDA)的超快速液相色谱(UFLC)方法对总酚酸(没食子酸、3,4二羟基苯甲酸、儿茶素、绿原酸、咖啡因、4羟基苯甲酸、表儿茶素、表没食子儿茶hingallate、咖啡酸、香草酸、丁香酸、芦丁、对香豆酸、香草素、辛酸、阿魏酸、rosemaric酸、连苯二甲酸、杨梅素、3,4-二羟基苯甲酸乙酯、木犀草素、槲皮素、反式肉桂酸、红枣果皮、果肉和种子酚类提取物中的柚皮素、芹菜素、山奈酚、橙皮素、ısorhamnetin、鼠李素、ladanein、pectolinaringenin、pinostrobin。果皮、果肉和种子总酚含量分别为27.16±1 55、33.74±0 60和10.37±0 86 mg没食子酸当量(GAE)/g干重。DPPH测定的抗氧化活性分别为62.32±0.79、122.63±0.92和15.52±0.80 mg特洛克斯当量(TE)/g干重。CUPRAC测定的抗氧化活性分别为60.62±1.92、69.91±0.92和21.03±1.73 mg Trolox (TE)/g干重。果皮、果肉和种子部分的花青素含量分别为419.97±10.89、29.41±6.45和24.05±5.18 mg。UFLC分析结果表明,儿茶素和香草酸是红枣各组分中主要的酚类物质。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Characterization of Phenolic Compounds in Peel, Pulp andSeed Fractions of Zizyphus Jujube Fruit by UFLC-PDA and Determination of Antioxidant Activity
The objective of this present study was to characterize peel, pulp and seed tissues of Zizyphus jujube, commonly known as «Jujube», to determine the total phenolic content, anthocyanin content, antioxidant activity and phenolic profile. Antioxidant potential of samples were measured with two different methods 2,2-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity and cupric reducing capacity (CUPRAC). Monomeric anthocyanin contents of the samples were determined using the pH differential method. The total phenolic content of the extracts was determined according to the Folin Ciocalteu method. Moreover, an ultra-fast liquid chromatograph (UFLC) method with photodiode array detector (PDA) was used to identify total phenolic acids (gallic acid, 3,4dihydroxybenzoic acid, catechin, chlorogenic acid, caffeinne, 4phydroxybenzoic acid, epicatechin, epigallocatechingallate, caffeic acid, vanilic acid, syringic acid, rutin, p-coumaric acid, vanilin, sinapic acid, ferulic acid, rosemarinic acid, phloridzin, myricetin, ethyl 3,4-dihydroxybenzoate, luteolin, quercetin, trans-cinnamic acid, naringenin, apigenin, kaempferol, hesperetin, ısorhamnetin, rhamnetin, ladanein, pectolinaringenin, pinostrobin) in peel, pulp and seed phenolic extracts of jujube. Total phenolic content of peel, pulp and seed were determined as 27.16±1 55, 33.74±0 60 and 10.37±0 86 mg gallic acid equivalent (GAE)/g dry weight, respectively. Antioxidant activities evaluated by DPPH were as follows 62.32±0 79, 122.63±0 92 and 15.52±0 80 mg Trolox equivalent (TE)/g dry weight, respectively. Antioxidant activities evaluated by CUPRAC were as follows 60.62±1.92, 69.91± 0.92 and 21.03±1.73 mg Trolox (TE)/g dry weight, respectively. Anthocyanin contents of the samples found as 419.97±10.89, 29.41±6.45 and 24.05±5.18 mg cyanidin 3-glucoside 100 g dry weight for peel, pulp and seed fractions, respectively. The results of UFLC analysis indicate that catechin and vanilic acid were the main phenolics in all fractions of jujube.
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