大黄黄芩合剂对急性反流性食管炎大鼠的影响

Jin A. Lee, Mi-Rae Shin, Sang-Nam Lee, Park, Soon-Ae, Hae-Jin Park
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引用次数: 3

摘要

目的:反流性食管炎是由胃内容物、胃酸和胃蛋白酶反流进入食管引起的疾病,目前在世界范围内呈增加趋势。研究了大黄黄芩合剂对大鼠急性反流性食管炎的治疗作用。方法:将大鼠分为5组:正常组(Nor, n=8)、水处理急性反流性食管炎大鼠(Con, n=8)、生育酚30 mg/kg体重/d处理急性反流性食管炎大鼠(Toco, n=8)、RS100 mg/kg体重/d处理急性反流性食管炎大鼠(RS100, n=8)、RS200 mg/kg体重/d处理急性反流性食管炎大鼠(RS200, n=8)。所有大鼠禁食18 h,然后通过连接幽门、前胃和体之间的转移连接处获得。术后5 h处死大鼠。采用western blot法分析大鼠食管组织中NADPH、MAPK、炎症、抗炎及紧密连接相关蛋白的表达,并观察血清中活性氧(ROS)、丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)水平。结果:RS给药对反流性食管炎食管黏膜损伤有显著保护作用,与Con组相比,RS给药组ROS、AST、ALT水平显著降低。此外,RS有效抑制了MAPK和NF-κB通路,上调了紧密连接蛋白的表达。结论:RS通过抑制MAPK和NF-κB通路,上调紧密连接,对食管黏膜具有保护作用。1)
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effect of a Mixture of Rhei Rhizoma and Scutellariae Radix Extract on Acute Reflux Esophagitis Rats
Objective : Reflux esophagitis is a disease caused by reflux of stomach contents, stomach acid, and pepsin into the esophagus, and is currently increasing worldwide. This study was conducted to evaluate the effect of a mixture of Rhei Rhizoma and Scutellariae Radix (RS) extract on acute reflux esophagitis in rats. Methods : Rats were divided into five groups for examination: Normal group (Nor, n=8), water-treated acute reflux esophagitis rats (Con, n=8), tocopherol 30 mg/kg body weight/day-treated acute reflux esophagitis rats (Toco, n=8), RS 100 mg/kg body weight/day-treated acute reflux esophagitis rats (RS100, n=8), RS 200 mg/kg body weight/day-treated acute reflux esophagitis rats (RS200, n=8). All rats fasted for 18 h and then were derived by linking the metastatic junction between pylorus and forestomach and corpus. And rats were sacrificed 5 h after surgery. We analyzed the expression of NADPH, MAPK, inflammatory, anti-inflammatory, and tight junction related proteins by western blot in esophageal tissue and observed the level of reactive oxygen species (ROS), alanine aminotransferanse (ALT), and aspartate aminotransferase (AST) in serum. Results : RS administration significantly protected the esophageal mucosal damage of reflux esophagitis, and ROS, AST, and ALT levels were significantly reduced in RS administration compared to Con group. In addition, RS administration effectively suppressed MAPK and NF-κB pathways and upregulated protein expressions of tight junction protein. Conclusions : These results suggest that RS protected the esophageal mucosa by inhibiting the MAPK and NF-κB pathways and upregulating tight junctions. 1)
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