能量饮料对大鼠大脑谷胱甘肽系统的影响

N. I. Lytvyniuk, A. Ersteniuk
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引用次数: 0

摘要

本文介绍了能量饮料对大鼠大脑谷胱甘肽系统状态影响的研究结果。能量补品是一组含有大量活性成分的饮料,这些活性成分能够刺激人的中枢神经系统,提高身体机能,并影响昼夜节律,延长清醒时间。文献资料也表明能量饮料对人体某些功能系统的负面影响。本研究以体重180-200 g的Wistar系大鼠为实验对象,在调节的微气候参数(湿度、光照和温度)下,饲喂标准的室内饲料。所有动物实验均按照《欧洲保护用于实验和科学目的的脊椎动物公约》(斯特拉斯堡,1986年)的要求进行。采用非吸入麻醉方法收集所需材料,即肌肉注射硫喷妥钠,剂量为60 mg/kg。按成样逻辑标准将实验动物分为5组:第一组(对照组)-大鼠饮水;2 -连续1个月给予能量饮料,在能量饮料饮用结束后第1天收集材料;第3组:连续饮用功能饮料1个月,实验结束后第10天收集材料;4 -动物连续1个月饮用能量饮料,实验结束后第20天收集材料;第5期:给大鼠灌能量饮料1个月,实验结束后第30天收集材料。采用酶法测定谷胱甘肽系统酶(谷胱甘肽还原酶、谷胱甘肽过氧化物酶和谷胱甘肽转移酶)和戊糖磷酸途径酶(葡萄糖-6-磷酸脱氢酶)的活性。实验动物群饮用能量饮料会导致谷胱甘肽系统酶活性的变化:第2、4、5组谷胱甘肽还原酶活性升高,第3组谷胱甘肽过氧化物酶活性降低,第4、5组谷胱甘肽过氧化物酶活性升高,第2、3组谷胱甘肽转移酶活性降低,所有实验动物谷胱甘肽转移酶活性均升高。葡萄糖-6-磷酸脱氢酶活性在2.3组中升高,在4.5组中降低。实验结果表明,能量饮料对实验动物脑组织的抗氧化保护状态,特别是对谷胱甘肽系统的状态有显著影响。酶活性指标的解释证明,能量组饮料可以导致进一步违反维持身体内环境稳定的能力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The state of the glutathione system of the cerebral of rats under the conditions of energy drink consumption
The paper presents the results of studies of the energy drink influence on the state of the glutathione system of the rat brain. Energy tonics belong to a group of drinks containing a large number of active components that are able to stimulate the central nervous system of a person and to increase physical performance, as well as to affect circadian rhythms, extending the period of wakefulness. Literary sources also indicate the negative impact of energy drinks on certain functional systems of the human body. The study was carried out on white rats of the Wistar line weighing 180–200 g, which were on a standard vivarium diet under regulated microclimate parameters (humidity, lighting and temperature regime). All experiments on animals were conducted in compliance with the requirements of the European Convention for the Protection of Vertebrate Animals Used for Experimental and Scientific Purposes (Strasbourg, 1986). A non-inhalation method of anesthesia was used to collect the necessary material, namely intramuscular injection of sodium thiopental at the rate of 60 mg/kg. The experimental animals were divided into 5 groups according to the logical criterion of sample formation: 1st (control group) — rats received drinking water; 2nd — the animals received an energy drink for a month, the material was collected on the 1st day after the end of energy drink consumption; 3rd — animals that received an energy drink for a month, the material was collected on the 10th day after the end of the experiment; 4th — the animals received an energy drink for a month, the material was collected on the 20th day after the end of the experiment; 5th — the rats received an energy drink for a month, the material was collected on the 30th day after the end of the experiment. Determination of the activity of enzymes of the glutathione system (glutathione reductase, glutathione peroxidase and glutathione transferase) and the enzyme of the pentose phosphate pathway (glucose-6-phosphate dehydrogenase) was performed using the enzymatic method. The consumption of an energy drink by experimental groups of animals leads to changes in the activity of enzymes of the glutathione system: an increase in the activity of glutathione reductase in the 2nd, 4th, 5th groups, at the same time as a decrease in the activity of this enzyme in representatives of the 3rd group of animals, the activity of glutathione peroxidase increased in the 4th, 5th and decreased in 2nd and 3rd groups, an increase in glutathione transferase activity was observed in all experimental animals. The activity of the enzyme glucose-6-phosphate dehydrogenase increased in 2.3 and decreased in 4.5 studied groups. The obtained results demonstrated a significant influence of the energy drink on the state of antioxidant protection of the brain tissues of experimental animals, in particular on the state of the glutathione system. The interpretation of enzyme activity indicators proves that energy group drinks can lead to further violations of the ability to maintain the stability of the body’s internal environment.
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