硝酸还原酶和亚硝酸盐还原酶在大叶藻耐盐性中的作用

X. Lv, Xinlei Wang, J. Pan, Wenhao Deng, Yuchun Li
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引用次数: 3

摘要

摘要硝酸盐还原酶(NR)和亚硝酸盐还原酶(NiR)在植物的硝酸盐同化中起着重要作用。已有研究表明,大叶藻的NR和NiR可能与大叶藻耐盐性有关。本研究研究了NR和NiR在大叶藻(Zostera marina)中的表达特性和生物学功能,并将其命名为ZmNR和ZmNiR,分别克隆、鉴定并在细菌和烟草中过表达。ZmNR和ZmNiR的开放阅读框包含2628和1773个核苷酸,分别编码875和590个氨基酸。氨基酸序列比对表明,所述ZmNR和ZmNiR蛋白与其他植物NR和NiR序列同源性较低。实时荧光定量PCR结果显示,在低盐度和高盐度诱导下,ZmNR和ZmNiR的表达均受到抑制。进一步的生理分析表明,添加Na2WO4阻断大叶藻对硝酸盐的同化,降低了大叶藻对NaCl胁迫的耐受性。ZmNR和ZmNiR基因在大肠杆菌和benthamiana中的异源表达可以产生对NaCl胁迫的抗性。生理和生长分析表明,ZmNR和ZmNiR通过调节一氧化氮(NO)引发的多种生理途径和生化过程来抵抗NaCl胁迫。综上所述,这些结果表明,依赖nr的NO合成可能在大叶藻耐盐性中起重要作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Role of Nitrate Reductase and Nitrite Reductase in NaCl Tolerance in Eelgrass (Zostera marina L.)
Abstract Nitrate reductase (NR) and nitrite reductase (NiR) play important roles in nitrate assimilation in plants. Previous studies have indicated that NR and NiR in eelgrass may contribute to its NaCl tolerance. This study investigated the expression characteristics and the biological functions of NR and NiR in eelgrass (Zostera marina), named as ZmNR and ZmNiR, were cloned, characterised and overexpressed in both bacteria and tobacco. The open reading frames of ZmNR and ZmNiR contain 2628 and 1773 nucleotides that encode 875 and 590 amino acids respectively. Amino acid sequence alignment indicated that the purported ZmNR and ZmNiR proteins presented low homology with other plant NR and NiR sequences. Real-time quantitative PCR revealed that the expression of ZmNR and ZmNiR was supressed when exposed to low salinity and induced by high salinity. Further physiological analyses demonstrated that blocking nitrate assimilation by adding Na2WO4 in eelgrass reduced its tolerance to NaCl stress. The heterologous expression of the ZmNR and ZmNiR genes in Escherichia coli and Nicotiana benthamiana could confer tolerance to NaCl stress. Physiological and growth analyses suggested that ZmNR and ZmNiR in plants could resist NaCl stress by regulating various physiological pathways and biochemical processes triggered by nitric oxide (NO). Taken together, these results suggested that NR-dependent NO synthesis may play an important role in NaCl tolerance in eelgrass.
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