孟加拉湾三种海藻的生物学研究:肠Enteromorpha enterinalis, Rhizoclonium riparium和Ceratophyllum demersum

Md Hanif Biswas, Nasiba Binte Bahar, N. Sharmin, Shiekh Zahir Raihan, S. Halder, Mir Shariful Islam, M. A. Muhit
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引用次数: 0

摘要

海洋由多种多样的生命领域组成,由于次生代谢物的多样性,可以为人类健康利益而加以利用。海藻,通常被称为大型藻类,常见于孟加拉湾的沿海地区。但它们的生物活性都没有得到适当的研究。因此,本研究采用标准方案对肠Enteromorpha ninteinalis、riparium rhizzoclonium riparium和Ceratophyllum demersum三种海藻粗甲醇提取物的抗氧化、抗菌、细胞毒和膜稳定活性以及总酚含量进行了研究。3种海藻的甲醇提取物中,金貂草(52.09±1.30 mg)的酚类含量最高,其次是肠棘草(35.98±1.27 mg),最后是河滨草(29.94±0.54 mg)。与标准品相比,这些海藻提取物也显示出良好的抗氧化和膜稳定性能。随着浓度的增加,肠菊提取物的还原力更强(EC50值为7.70±0.27 μg/ml)。与抗坏血酸的IC50值(5.76±0.13 μg/ml)相比,同一种海藻对DPPH的清除能力(IC50值为23.46±0.54 μg/ml)显著提高。然而,抑菌活性试验结果表明,这些海藻对大肠杆菌和黑曲霉有轻微的抑菌作用,对HeLa细胞株没有细胞毒性。这些发现将有助于未来试图从孟加拉国这些现成的海藻中探索药物和治疗剂的研究。达卡大学药学院。自然科学22(1):81- 87,2023 (6)
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Biological Investigations of Three Marine Algae Enteromorpha intestinalis, Rhizoclonium riparium and Ceratophyllum demersum Collected from the Bay of Bengal
The ocean consists of versatile domain of lives which can be exploited for human health benefits because of diversified secondary metabolites. Seaweeds, generally called macroalgae, are commonly found in the coastal region of the Bay of Bengal. But none of them were investigated properly for their biological activities. Hence, this study was performed to investigate the antioxidant, antimicrobial, cytotoxic and membrane stabilizing activities along with total phenolic content of the crude methanolic extract of three seaweeds namely, Enteromorpha intestinalis, Rhizoclonium riparium and Ceratophyllum demersum using standard protocols. Among the methanolic extracts of three seaweeds, C. demersum (52.09 ± 1.30 mg of GAE) was found to have the highest phenolic content, followed by E. intestinalis (35.98 ± 1.27 mg of GAE) and then R. riparium (29.94 ± 0.54 mg of GAE). The extractives of these seaweeds also showed promising antioxidant and membrane stabilizing properties compared to the standard. E. intestinalis extract displayed greater reducing power with increasing concentration (EC50 value 7.70 ± 0.27 μg/ml). The same seaweed E. intestinalis was found to have significant free radical (DPPH) scavenging potential (IC50 value 23.46 ± 0.54 μg/ml) compared to the reference standard ascorbic acid (IC50 value 5.76 ±0.13 μg/ml). However, the findings of antimicrobial activity test demonstrated mild antibacterial effects of these seaweeds against Escherichia coli and Aspergillus niger and none of these seaweeds exhibited cytotoxicity when tested against HeLa cell line. These findings will aid in future studies attempting to explore medicinal and therapeutic agents from these readily available seaweeds of Bangladesh. Dhaka Univ. J. Pharm. Sci. 22(1): 81-87, 2023 (June)
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