Comparing 16S rRNA gene similarity with simple polar lipids profiling amongst Salmonella isolates

Background and Objectives: Polar lipids and the 16S rRNA gene have a significant role in taxonomic characteristics. The study's objective is to determine the potential relationship between simple migration distances (mm) of polar lipids and Salmonella's 16S rRNA gene similarity. Materials and Methods: Based on 16S RNA sequences and simple thin-layer chromatography migration distances (mm) of polar lipids, allowed to compare the various Salmonellae species, and apply it to examine the variability and estimate bacterial similarities. Results: The chromatography migration distance analysis revealed 3–4 spots of polar lipids. The polar lipids revealed two denser spots, the most abundant lipids, between 10 and 28 mm. The other two low-density spots of migration distance ranged in size from 23 to 25 mm. Between 99.4% and 100% of the three Salmonella isolates and other Salmonella species exhibited 16S rRNA similarities. One strain had a similarity of 98.9%. These findings demonstrated the nearly identical 16S rRNA gene sequences and polar lipids profile of the isolates. Conclusions: This study has concluded that all Salmonella species share similarities in both the polar lipid profiles and the 16S rRNA genes. The study validates the utility of coupling of polar lipids and 16S rRNA gene sequencing as useful tools for taxonomic differentiation and epidemiological tracing of Salmonella.