Ahmad Musa, Rémi Dugué, Rina Hirnawati, Bastiar Nur, Rendy Ginanjar, Sawung, Cindelaras, S. Sugito
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摘要

龙鱼是一种单形物种,这些动物在生理上无法区分雌雄。本研究旨在鉴定和纯化班加尔、巴布亚、皮诺和超级红四种亚洲龙鱼的卵黄蛋白原。12条鱼,每种3条,通过蟾蜍刺激雌二醇产生卵黄原素。SDS-PAGE结果表明,亚洲龙鱼卵黄原蛋白存在两种类型,其中Banjar龙鱼、Pinoh (macrocephalus)和Super Red (Scleropages legendrei)为单Vtg,分子量为180 kDa;巴布亚龙鱼(Scleropages jardinii)为双Vtg,分子量为180和110 kDa。从班贾尔、巴布亚和超级红3个品种中采集到纯卵黄原蛋白,浓度范围在0.1 - 0.67 mg / mL之间,实际浓度应该大于测定浓度。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Identifikasi dan Purifikasi Vitelogenin Arwana Asia: Banjar, Pinoh (Scleropages macrocephalus), Papua (Scleropages jardinii) dan Super Red (Scleropages legendrei)
Arowana fish (Scleropages sp.) are monomorphic species, those animals that physically could not be distinguished between male and female. The research was aimed to identify and purify Vitellogenin of four variants of Asian Arowana: Banjar, Papua, Pinoh, and Super red. 12 fishes, 3 from each variant were given estradiol stimulation through toad for vitellogenin production purpose. The SDS-PAGE results expressed that there were two types of Asian Arowana vitellogenin characters, single Vtg for Arowana Banjar, Pinoh (Scleropages macrocephalus) and Super Red (Scleropages legendrei) with a molecular weight of 180 kDa and double Vtg in Papuan Arowana (Scleropages jardinii) with molecular weight 180 and 110 kDa. Pure vitellogenin has been collected from 3 varieties, Banjar, Papua, and Super red with a concentration range between 0.1 - 0.67 mg / mL. The actual concentration is believed to be greater than the measured concentration.
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