含铜胺氧化酶参与豚鼠卵清蛋白诱导支气管哮喘肺病理的发展

O. Hudkova, S. Luhovskyi, L. Drobot, N. Latyshko
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引用次数: 0

摘要

支气管哮喘是一种对过敏原挑战的免疫反应,伴随着气道重构中的炎症和纤维化。为了揭示含铜氨基氧化酶氨基脲敏感胺氧化酶(SSAO)、氨基氧化酶(DAO)和赖氨酸氧化酶(LOX)在BA发育中的致病作用,我们利用它们的不可逆抑制剂氨基脲和卵清蛋白诱导BA的豚鼠模型。氨基脲通过饮水或吸入给药给哮喘动物。诱导后第16周,观察到血浆促炎SSAO和DAO活性升高(分别为1.6倍和2倍)。与未治疗的哮喘动物相比,通过饮用或吸入氨基脲显著降低了这些酶的活性。观察到哮喘动物肺组织中IL-13含量和LOX活性显著增加,证明气道炎症和肺纤维化的发生。豚鼠支气管哮喘对氨基脲的摄取导致LOX活性正常化。组织学研究证实,氨基脲可减轻哮喘动物肺部的形态病理变化。因此,所获得的数据表明,所研究的酶直接参与特应性支气管哮喘的病理过程的进展,以及氨基脲作为一种药物在复杂的抗哮喘治疗中的潜在应用。关键词:特应性支气管哮喘,组胺酶/二胺氧化酶,IL-13,赖氨酸氧化酶,一氧化氮,氨基脲,氨基脲敏感胺氧化酶
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Involvement of Cu-containing amine oxidases in the development of lung pathology in ovalbumin-induced bronchial asthma in guinea pigs
Bronchial asthma is developed as an immune response to allergen challenges accompanied by inflammation and fibrosis implicated in airway remodeling. To reveal the causative implication of Cu-containing amino oxidases semicarbazide-sensitive amine oxidase (SSAO), DAO and lysyl oxidase (LOX) in BA development we used their irreversible inhibitor semicarbazide and guinea pig model of BA induced by ovalbumin. Semicarbazide was introduced to asthmatic animals via drink or inhalation. At the 16th week after disease induction, the increase in the activity of pro-inflammatory SSAO and DAO in plasma (1.6 and 2 times, respectively) was observed. The introduction of semicarbazide to asthmatic animals via drink or inhalation significantly decreased activities of these enzymes compared to the untreated asthmatic animals. A considerable­ increase in IL-13 content and LOX activity in the lung tissue of asthmatic animals were observed that evidenced airway inflammation and pulmonary fibrosis development. The uptake of semicarbazide by guinea pigs with bronchial asthma led to normalization of LOX activity. Histological studies confirmed that semicarbazide attenuated morphopathological changes in the lungs of asthmatic animals. Thus, the data obtained indicate the direct participation of the studied enzymes in the progression of pathological processes in atopic bronchial asthma as well as the potential use of semicarbazide as a drug in complex anti-asthmatic therapy. Keywords: atopic bronchial asthma, histaminase/diamine oxidase, IL-13, lysyl oxidase, nitric oxide, semicarbazide, semicarbazide sensitive amine oxidase
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