中药复方提取物对小鼠的镇痛和抗炎作用

M. Chien, C. Chuang, Yi-Ting Lin, Chih-Min Yang, Chao-Hsiang Chen
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引用次数: 0

摘要

由大豆精、蛇床子、甘草和鹿角组成的混合提取物。本实验采用热板实验、醋酸(AA)诱导扭体实验和醋酸诱导血管通透性实验三种小鼠模型,研究HME的镇痛和抗炎作用。结果显示,HME在热板实验和aa扭体实验中均表现出镇痛作用,分别表现为增加舔爪潜伏期和减少aa扭体次数。HME还能显著且剂量依赖性地降低aa诱导的血管通透性,表明HME具有抗炎作用。在本研究中,作为阳性对照的阿司匹林治疗也可以观察到类似的改善。蛇床子的大部分药理作用被认为归因于香豆素,如蛇床子酚(OST)和欧前胡素(IMP)具有多种药理活性。然后我们将OST和IMP作为HME的生物活性成分。三个独立批次的HME中OST和IMP的含量分别为3.57±0.10 mg/g和1.20±0.02 mg/g。在三种小鼠模型中,只有OST具有抑制作用,提示OST可能部分参与了HME的保护作用。这些结果表明,HME具有潜在的抗镇痛和抗炎作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Analgesic and Anti-Inflammatory Effects of Herbal Mixture Extracts in Mice
Herbal mixture extracts (HME) comprised of Semen Sojae Nigrum, Fructus Cnidii, Radix Glycyrrhizae, and Cornu Cervi. Herein, we employed three mouse models, including hot-plate test, acetic acid (AA)-induced writhing test and AA-induced vascular permeability test, to determine analgesic and anti-inflammatory effects of HME. Results revealed that HME exhibited analgesic effects in hot-plate test and in AA-induced writhing test, as evidenced by increasing the latency to lick paws and decreasing AA-induced writhing counts, respectively. HME also significantly and dose-dependently decreased AA-induced vascular permeability, indicating HME exhibited anti-inflammatory effects. Similar improvement can be observed in aspirin treatment that used as positive control in this study. Most of medicinal effects of Fructus Cnidii are considered to attribute to coumarins, such as osthol (OST) and imperatorin (IMP) with several pharmacological activities. We then used OST and IMP as bioactive components in HME. The content of OST and IMP in HME was 3.57 ± 0.10 mg/g and 1.20 ± 0.02 mg/g, respectively, from three independent batches. Only OST possessed inhibitory effects in three mouse models, suggesting that OST may partially involve in protective effects of HME. These results demonstrated that HME has a potential on anti-analgesic effects and anti-inflammatory actions.
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