五倍子与不同抗菌药物联合对耐碳青霉烯大肠杆菌的体外抑菌活性研究

Zhizhi Xie, Changzhi Xu, Yanhua Yi, Donglin Zhu, Yun Xi
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引用次数: 0

摘要

目的:评价美罗培南和左氧氟沙星分别联合五倍子对耐碳青霉烯大肠杆菌的体外抑菌效果。方法:采用棋盘法设计方案,在我院分离出耐碳青霉烯类大肠杆菌。采用肉汤稀释法测定羊草单用及联用2种抗菌药物对耐碳青霉烯类大肠杆菌的最低抑菌浓度(mic),并根据mic结果计算分式抑菌浓度指数(FICI)。结果:羊草与美罗培南(或左氧氟沙星)联用可显著降低MIC50和MIC90;与美罗培南联用后,协同效应为86.7%,加性效应为13.3%,无无关效应和拮抗效应。五味子与左氧氟沙星联合使用后,协同效应为66.7%,加性效应为33.3%。无无关和拮抗作用。结论:五倍子与美罗培南、左氧氟沙星联用具有增效和加性抑菌作用,具有一定的联合抑菌活性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
In Vitro Antibacterial Activity of Galla Chinensis Combined with Different Antibacterial Drugs against Carbapenem-Resistant E.Coli
Objective: To evaluate the antibacterial effects of meropenem and levofloxacin respectively combined with Galla chinensis on carbapenem-resistant Escherichia coli in vitro. Methods: The protocol was designed with checkerboard method and the carbapenem-resistant E.coli was isolated in our hospital. The minimum inhibitory concentrations(MICs) of G. chinensis alone and combined with 2 antimicrobial agents against carbapenem-resistant E.coli were determined by broth dilution method and the fractional inhibitory concentration index (FICI) was calculated according to MICs results. Result: the combined use of G. chinensis and meropenem (or levofloxacin) significantly decreased both MIC50 and MIC90; After the combination of G. chinensis and meropenem, the synergistic effect was 86.7%, and the additive effect was 13.3%, no irrelevant and antagonistic effects. After combined use of G. chinensis and levofloxacin, the synergistic effect was 66.7%, and the additive effect was 33.3%. No irrelevant and antagonistic effects. Conclusion: Galla chinensis combined with meropenem or levofloxacin has synergistic and additive antibacterial effect, with certain combined antibacterial activity.
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