设计微氧诱导型 miniR1 质粒。

IF 1.7 3区工程技术 Q3 ENGINEERING, CIVIL

Journal of Hydraulic Research Pub Date : 2023-03-20 eCollection Date: 2023-03-01 DOI:10.1002/mlf2.12058

Fabiola Islas, Andrea Sabido, Juan-Carlos Sigala, Alvaro R Lara

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引用次数: 0

摘要

制造质粒 DNA 是生产基因治疗药物和下一代疫苗的必要步骤。然而，人们很少关注开发可与大规模生产条件相结合的替代复制子。我们的研究结果表明，当使用受微需氧启动子控制的调控蛋白 RepA 复合物时，miniR1 复制子可在氧限制条件下被有效诱导。这些结果对工业应用具有潜在的吸引力。

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Design of microaerobically inducible miniR1 plasmids.

Plasmid DNA manufacture is an essential step to produce gene therapy agents and next-generation vaccines. However, little attention has been paid toward developing alternative replicons that can be coupled with large-scale production conditions. Our results demonstrate that the miniR1 replicon can be efficiently induced by oxygen limitation when a copy of the regulatory protein RepA under control of a microaerobic promoter is used. The results are potentially attractive for industrial applications.

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来源期刊

Journal of Hydraulic Research 工程技术-工程：土木

CiteScore

4.90

自引率

4.30%

发文量

审稿时长

6.6 months

期刊介绍： The Journal of Hydraulic Research (JHR) is the flagship journal of the International Association for Hydro-Environment Engineering and Research (IAHR). It publishes research papers in theoretical, experimental and computational hydraulics and fluid mechanics, particularly relating to rivers, lakes, estuaries, coasts, constructed waterways, and some internal flows such as pipe flows. To reflect current tendencies in water research, outcomes of interdisciplinary hydro-environment studies with a strong fluid mechanical component are especially invited. Although the preference is given to the fundamental issues, the papers focusing on important unconventional or emerging applications of broad interest are also welcome.