卡诺州达瓦瑙谷物市场玉米样品伏马菌素含量的研究

Shamsuddeen U., Kabir A.
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引用次数: 2

摘要

对卡诺州达瓦瑙谷物市场玉米的黄曲霉毒素含量进行了研究。共采集25份玉米样品,进行水分含量测定、霉菌分离、黄曲霉毒素提取和毒素分析。25份玉米样品中分别有9份(36%)、6份(24%)、19份(76%)、7份(28%)、4份(16%)、8份(32%)、2份(8%)和4份(8%)检出黄霉、寄生、镰刀菌、黑霉、青霉、毛霉、黑孢子菌和木霉。薄层色谱法对提取物进行分析,紫外光照射后,在薄层色谱板上产生蓝、蓝绿色荧光,荧光系数在0.40 ~ 0.65之间。酶联免疫吸附测定结果表明,黄曲霉毒素含量在22.05 ~ 44.56之间。该研究强调有必要让公众了解与食用真菌毒素有关的危险和潜在危害。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Study on Fumonisin Contents of Maize Sampled From Dawanau Grains Market in Kano
A study was carried out on the Aflatoxin content of maize sourced from Dawanau grains market in Kano. A total of 25 maize samples were collected and subjected to moisture content determination, moulds isolation as well as aflatoxin extraction which were followed by the analysis of the toxin. Of the 25 maize samples 9 (36%), 6 (24%), 19 (76%), 7 (28%), 4 (16%), 8 (32%), 2 (8%) and 4 (8%) yielded A. flavus, A. parasiticus, Fusarium, A. niger, Penicillium, Mucor, NigrosporaandTrichodermarespectively. Analysis of the extracts using thin layer chromatography yielded blue and blue green fluorescence on the TLC plate after U.V light illumination withRf values which ranged between 0.40 – 0.65. Analysis of the extracts using ELISA kit showed the aflatoxin content of the maize to be in the range of 22.05 – 44.56. The study stressed the need for public enlightenment on the dangers and potential hazards associated with the consumption of mycotoxins.
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