利用肽标记对考古蛋壳进行鉴定

S. Presslee, Julie Wilson, Jos Woolley, Julia Best, D. Russell, A. Radini, R. Fischer, B. Kessler, R. Boano, M. Collins, B. Demarchi
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引用次数: 31

摘要

禽蛋壳在碱性和中性土壤中生存良好,但其作为考古资源的潜力尚未得到充分开发,主要原因是其鉴定困难。在此,我们利用新的鸟类基因组的释放,并首次在蛋壳上使用MALDI-ToF(基质辅助激光解吸电离飞行时间)质谱法结合LC-MS/MS的肽测序。蛋壳蛋白质组出人意料地复杂,在包含23种鸟类的参考集合中鉴定出5755种蛋白质。我们确定了782个可用于蛋壳鉴定的m/z标记,其中583个可分配给已知的蛋壳肽序列。这些被用来鉴定从莱斯特的自由学校巷的中世纪遗址中发现的蛋壳碎片。我们讨论了肽标记的特异性,并强调了评估考古解释可实现的分类鉴定水平的重要性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The identification of archaeological eggshell using peptide markers
ABSTRACT Avian eggshell survives well in alkaline and neutral soils, but its potential as an archaeological resource remains largely unexplored, mainly due to difficulties in its identification. Here we exploit the release of novel bird genomes and, for the first time on eggshell, use MALDI-ToF (matrix-assisted laser desorption ionisation-time of flight) mass spectrometry in combination with peptide sequencing by LC-MS/MS. The eggshell proteome is revealed as unexpectedly complex, with 5755 proteins identified for a reference collection comprising 23 bird species. We determined 782 m/z markers useful for eggshell identification, 583 of which could be assigned to known eggshell peptide sequences. These were used to identify eggshell fragments recovered from a medieval site at Freeschool Lane, Leicester. We discuss the specificity of the peptide markers and highlight the importance of assessing the level of taxonomic identification achievable for archaeological interpretation.
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