hplc -MS/MS ESI和APC法研究17b-雌二醇体外代谢

Analusis Pub Date : 2000-06-01 DOI:10.1051/ANALUSIS:2000280273

E. Rathahao, A. Hillenweck, A. Paris, L. Debrauwer

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引用次数: 12

摘要

采用电喷雾电离(ESI)和常压化学电离(APCI)相结合的液相色谱-串联质谱法研究了17β-雌二醇在大鼠离体肝细胞中的体外代谢。17β-雌二醇和雌酮对应的儿茶酚类雌激素通过ESI及其谷胱甘肽和糖甙缀合物进行了鉴定，而更多的极性化合物，如甲氧基儿茶酚类雌激素则通过APCI进行了表征。

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Investigation of the in vitro metabolism of 17b-estradiol by LC-MS/MS using ESI and APC

Liquid chromatography coupled to tandem mass spectrometry with electrospray ionisation (ESI) and atmospheric pressure chemical ionisation (APCI) were used for the investigation of the in vitro metabolism of 17β-estradiol on rat isolated hepatocytes. The catechol estrogens corresponding to 17β-estradiol and estrone were identified by ESI, together with their glutathione and glucoronide conjugates, whereas more apolar compounds such as methoxylated catechol estrogens were characterised using APCI.

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Analusis

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