海绵体真菌长尾木霉提取物对病原菌的抑菌活性研究

Q3 Environmental Science
S. Sedjati, A. Ambariyanto, A. Trianto, E. Supriyantini, A. Ridlo, M. Bahry, Gita Wismayanti, O. Radjasa, Erin P. McCauley
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引用次数: 1

摘要

本研究旨在探索从印尼东部北马鲁古岛Ternate水域分离的一种海绵相关真菌长尾achiatum木霉的抗菌潜力。用不同的培养基刺激长尾藤次生代谢物的产生。分离物在各种培养基中培养6-9天。测定乙酸乙酯提取物对耐多药病原菌(铜绿假单胞菌、金黄色葡萄球菌、肺炎克雷伯菌和蜡样芽孢杆菌)的抑菌活性。结果表明,所有提取物在薄层色谱上具有相似的特征。然而,两种最有效的提取物是从PCA和MEA培养基中提取的,时间为9天。这些提取物抑制耐甲氧西林金黄色葡萄球菌(MRSA) (12.48 mm和12.27 mm);蜡样芽孢杆菌(12.11 mm和12.12 mm);肺炎克雷伯菌(12.40 mm和10.76 mm);P. aeruginosa (11.59 mm和8.69 mm)浓度为500 mg/盘。综上所述,在PCA和MEA培养基中培养的长尾曲霉具有产生耐多药病原菌抗菌化合物的潜力,且两者具有相似的抗菌化合物。同时,在TPA和TA培养基中培养的真菌乙酸乙酯提取物对所有被试细菌均无活性
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Antibacterial Activities of the Extracts of Sponge-Associated Fungus Trichoderma longibrachiatum against Pathogenic Bacteria
This study aims to explore the antibacterial potential of a sponge-associated fungus Trichoderma longibrachiatum isolated from Ternate waters, North Maluku, Eastern Indonesia. Various culture media were used to stimulate the production of secondary metabolites in T. longibrachiatum. The isolate was cultured in various media for 6-9 days. Then, the antibacterial activities of the ethyl acetate extracts were assayed against pathogenic bacteria of Multi-Drug Resistant (MDR) strain (Pseudomonas aeruginosa, Staphylococcus aureus, Klebsiella pneumoniae, and Bacillus cereus). The results showed that all extracts had similar profiles on the thin layer chromatography. However, two of the most potent extracts were produced from the PCA and MEA media for 9 days. These extracts inhibited methicillin-resistant S. aureus (MRSA) (12.48 mm and 12.27 mm); B. cereus (12.11 mm and 12.12 mm); K. pneumoniae (12.40 mm and 10.76 mm); and P. aeruginosa (11.59 mm and 8.69 mm) at concentrations 500 mg/disc. In conclusion, the fungus T. longibrachiatum that was cultured in PCA and MEA media had the potential to produce antibacterial compounds against MDR pathogens and both had similar compounds.  Meanwhile, the  ethyl acetate extracts from fungus cultured in the TPA and TA media were inactive against all tested bacteria
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来源期刊
CiteScore
1.40
自引率
0.00%
发文量
10
审稿时长
16 weeks
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