苹果根际根际分离菌的生防和促生活性筛选

Salma Jabiri, Ikram Legrifi, Majda Benhammou, Salah-Eddine Laasli, F. Mokrini, M. B. Amraoui, R. Lahlali
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引用次数: 1

摘要

苹果作物容易受到几种限制其产量的疾病——特别是由一种新的卵菌属引起的根腐病,主要是涡旋菌。本研究旨在筛选在该致病卵菌的生物防治中发挥重要作用的拮抗菌,并评价其促进植物生长的能力。双重培养试验表明,在200株分离菌中,有16株能够抑制vexans的菌丝生长,抑制率大于50%。根据16S rDNA基因对所选菌株进行鉴定:14株细菌隶属于芽孢杆菌属、窄养单胞菌属和肠杆菌科。值得注意的是,两个分离株B1和M2-6(鉴定为velezensis)的抑制率最高,分别为70%和68%。对这些选定的分离株进行了检测,以确定它们产生与生物防治和促进植物生长有关的不同化合物的能力。此外,我们还对这16株分离菌株在体内产生与生物防治和促进植物生长有关的化合物的能力进行了评估,这些化合物包括水解酶(纤维素酶、蛋白酶和淀粉酶)、HCN(氰化氢)生成、磷酸盐增溶、IAA(吲哚-3-乙酸)生成、果胶酶生成和刺激高粱双色生长。不同的细菌分离物在化合物生产和植物刺激能力方面存在差异。然而,在所有拮抗菌株中均检测到蛋白酶的分泌。使用聚合酶链反应(PCR)技术确定了在所选细菌分离株中负责生产抗真菌脂肽(bacillomycin, fengycin和iturin)的基因的存在,而通过PCR研究也证实了参与表面素生物合成的基因的缺失。这些菌株通过产生蛋白酶和抗真菌脂肽表现出抑制活性。需要进一步的研究来探索它们在生物防治策略和提高苹果作物产量方面的潜在应用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Screening of Rhizobacterial Isolates from Apple Rhizosphere for Their Biocontrol and Plant Growth Promotion Activity
Apple crops are prone to several diseases that limit their production—in particular, root rot caused by a new genus of oomycetes, mainly Phytopythium vexans. This study aims to screen antagonistic bacteria that can play an important role in the biological control of this pathogenic oomycete and to evaluate their capacity to promote plant growth. The dual culture test revealed that, out of 200 bacterial isolates, 16 have been able to inhibit the mycelial growth of P. vexans with inhibition rates greater than 50%. The selected isolates were identified based on the 16S rDNA genes: 14 bacteria belonging to the genus Bacillus, Stenotrophomonas, and the family Enterobacteriaceae. Notably, two isolates, B1 and M2-6 (identified as Bacillus velezensis), demonstrated the highest inhibition rates of 70% and 68%, respectively. These selected isolates were examined for their ability to produce different compounds related to biocontrol and plant growth promotion. Furthermore, the 16 selected isolates were evaluated for their ability to produce compounds associated with biocontrol and plant growth promotion, including hydrolytic enzymes (cellulases, proteases, and amylases), HCN (hydrogen cyanide) production, phosphate solubilization, IAA (indole-3-acetic acid) production, pectinase production, and stimulation of sorghum bicolor growth in vivo. Variations were observed among the bacterial isolates in terms of their compound production and phytostimulation capabilities. However, the secretion of proteases was consistently detected in all antagonistic isolates. The presence of genes responsible for the production of antifungal lipopeptides (bacillomycin, fengycin, and iturin) in the selected bacterial isolates was determined using polymerase chain reaction (PCR) techniques, while the absence of genes involved in surfactin biosynthesis was also confirmed through PCR studies. These isolates demonstrated inhibitory activity through the production of proteases and antifungal lipopeptides. Further research is needed to explore their potential use in biological control strategies and to improve apple crop productivity.
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