从红藻褐藻和尾藻生物量中提取和定量杂交角叉菜胶

R. Tuvikene, K. Truus, M. Vaher, T. Kailas, P. Kersen
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引用次数: 49

摘要

定量研究了从爱沙尼亚波罗的海Kassari藻层海藻中分离杂化卡拉胶共混物的主要工艺流程(提取、碱改性和沉淀)。在使用所有提取剂(水,KOH和NaOH溶液)的情况下,在前2小时内提取率急剧上升。纯水是从毛曲霉-尾形虫生物量中提取卡拉胶的最有效的提取介质,但提取的多糖的凝胶性(凝胶强度)肯定不是。长时间碱浸对角叉菜胶的降解造成很大的损失;然而,碱处理是从卡萨里藻层中分离高质量胶凝半乳的必要步骤,导致凝胶强度增加3倍以上。从该地层中提取的典型海藻混合物(69%的蓝藻和10%的长角藻)中,分离卡拉胶混合物的最有效培养基为0.05 M KOH溶液,最佳提取时间为3-4小时。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Extraction and quantification of hybrid carrageenans from the biomass of the red algae Furcellaria lumbricalis and Coccotylus truncatus
The main isolation procedures (extraction, alkali modification, and precipitation) of hybrid carrageenan blend from seaweeds of the Kassari algal stratum (the Baltic Sea, Estonia) were quantitatively investigated. In the case of all extracting agents (water, KOH and NaOH solutions) used, a sharp rise occurred in the extracting rate during the first 2 h. Pure water was the most efficient extracting medium for carrageenans from the biomass of Furcellaria lumbricalis- Coccotylus truncatus with regard to yield but certainly not gelling properties (gel strength) of polysaccharides extracted. Long-time alkali extraction causes great losses due to degradation of carrageenans; however, alkali treatment is an obligatory step for the isolation of high-quality gelling galactans from the Kassari algal stratum, resulting in a more than 3-fold increase in gel strength. In the case of a typical algal blend from this stratum (69% F. lumbricalis and 10% C. truncatus), the most effective medium for the isolation of carrageenan mixture is 0.05 M KOH solution and the preferred duration of extraction is 3-4 h.
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