越南红河三角洲地区市售猪肉和鸡肉中抗生素残留水平的初步评价。

P. Kim, G. Degand, C. Douny, G. Pierret, P. Delahaut, T. V. Dinh, B. Granier, M. Scippo
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引用次数: 24

摘要

从越南红河三角洲3个代表性省份(河内、海阳和泰平)随机抽取97份猪肉和83份鸡肉样品,采用两种微生物学方法(Premi®-test和New Two Plate Test)进行筛选。总共有27%的样品在两种测试中至少有一种表现出积极反应,其中11种(13%的鸡肉样品)是鸡肉样品,38种(39%的猪肉样品)是猪肉样品。筛选阳性的33份Thai Binh样品随后提交进行四环素和(氟)喹诺酮类抗生素特异性筛选后试验(四环素的tetrassensor®试纸和喹诺酮类的ELISA),这两组抗生素在该地区广泛用于动物生产,并通过液相色谱-质谱联用法确认。通过筛选后试验,分别在23份和5份样品中发现了四环素和(氟)喹诺酮类药物残留。10份(全部猪肉)和4份(1份猪肉,3份鸡肉)分别检出四环素类药物(氯四环素、六环素、四环素、六环素)和(氟)喹诺酮类药物(萘啶酸、恩诺沙星和环丙沙星),其中1份猪肉和3份猪肉样品分别检出恩诺沙星和四环素残留,其浓度均高于各自的最大限量。本研究表明,所提出的识别非相容性肉类样品的策略(微生物筛选,四环素和喹诺酮类靶向后筛选和确认)具有良好的性能,可以在82%的病例中获得结结性结果。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Preliminary evaluation of antimicrobial residue levels in marketed pork and chicken meat in the Red River Delta region of Vietnam.
The presence of antibacterial in 97 pork and 83 chicken meat samples, rando mly collected fro m 3 different representative provinces (Hanoi, Hai Duong and Thai Binh) of the Red River Delta, was determined by a screening step using in parallel 2 microbiological methods (Premi ® -test and New Two Plate Test). In total, 27% of all samples displayed a positive response in at least one of both tests, from which 11 (13% of chicken samples) are ch icken samples and 38 (39% of pork samples) are pork samples. The 33 samples fro m the Thai Binh which were screened positive were then submitted to post-screening tests specific for tetracyclines and (fluoro) quinolones (Tetrasensor ® dipstick for tetracyclines and an ELISA for quinolones), two groups of antibiotics widely used in animal production in this region, and confirmed by liquid chromatography coupled to mass spectrometry. Tetracyclines and (fluoro)quinolones residues were found, using a post screening test, in 23 and 5 samp les, respectively. Ten (all pork) and 4 samples (1 pork, 3 chicken) were confirmed containing tetracyclines (chlortetracycline, o xytetracycline, tetracycline, do xycycline) and (fluoro) quinolones (nalidixic acid, enroflo xacin and ciproflo xacin) respectively, fro m which 1 and 3 pork samples were found to contain enrofloxacin and tetracycline residues , respectively, with a concentration higher than their respective MRLs. This study shows the good performance of the proposed strategy to identify non-co mpliant meat samples (microbiological screening, tetracyclines and quinolones targeted post-screening and confirmation), which allows to obtain conclusive results in 82% of the cases.
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