氟对中枢神经系统钙/钙调素依赖性蛋白激酶- II mRNA和蛋白表达的影响

Q3 Medicine

中华地方病学杂志 Pub Date : 2011-05-20 DOI:10.3760/CMA.J.ISSN.1000-4955.2011.03.004

Jin Ting-xu, Guang Zhi-zhong, Z. Hua

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In post-weaning period, another 8 pups in each group were given drinking water with the same dose of fluoride as their mother's 21 day after birth. After then, these pups were killed on day 28, and hippocampus was dissected immediately and Western blot was conducted mRNA and protein levels were decreased. When NaF concentrations were 0,0.05,0.50,2.00,5.00 mmol/L, the mRNA relative ratios of α-CaMKⅡ in SY5Y cells were 1.00 ± 0.00,0.77 ± 0.18,0.40 ± 0.11,0.22 ± 0.06 and 0.15 ± 0.03, and protein levels of α-CaMK Ⅱ were 100.00 ± 0.00,76.17 ± 2.08,59.16 ± 2.12,48.52 ± 2.71 and 43.51 ± 2.57, any mmol/L group, hippocampus α-CaMK Ⅱ protein levels on day 14 and 28(75.02 ± 2.88,73.83 ± 3.88 and 81.00 ± 2.54,45.70 ± 2.34) were significantly lower than that of control groups(100.00 ± 0.00,100.00 ± 0.00, all P < 0.01). In 3 mmol/L group, hippocampus α-CaMKⅡ protein level on day 28 was lower than that of 2 mmol/L group (P < 0.01). 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引用次数: 2

摘要

目的研究氟对钙/钙调素依赖性蛋白激酶-Ⅱ(α-CaMKⅡ)亚基mRNA和蛋白表达的影响，并将终浓度分别为NaF 0(对照)、0.05、0.50、2.00、5.00 mmol/L的人神经母细胞瘤细胞置于DMEM中培养48 h。采用定量RT-PCR和Western blot检测α-CaMKⅡP1(出生后第1天)的表达水平，将幼鼠与母鼠随机分为3组。给哺乳期大鼠饮用浓度分别为0(对照)、2、3 mmol/L的NaF水。幼崽通过牛奶接触到NaF。每组在出生后第14天处死幼崽8只。断奶后，每组8只幼崽在出生后21天饮用与母鼠相同剂量的氟化物水。第28天处死，立即解剖海马，进行Western blot，降低mRNA和蛋白水平。氟化钠浓度0时,0.05,0.50,2.00,5.00更易/ L, mRNA的相对比例α-CaMKⅡSY5Y细胞分别为1.00±0.00、0.77±0.18、0.40±0.11、0.22±0.06、0.15±0.03,和蛋白质水平的α-CaMKⅡ分别为100.00±0.00、76.17±2.08、59.16±2.12、48.52±2.71、43.51±2.57,更易与L组,海马α-CaMKⅡ蛋白质含量14天、28天(75.02±2.88,73.83±3.88,81.00±2.54,45.70±2.34)明显低于对照组(100.00±0.00,100.00±0.00,P < 0.01)。3 mmol/L组28 d海马α-CaMKⅡ蛋白水平低于2 mmol/L组(P < 0.01)。结论氟可降低神经细胞和海马α-CaMKⅡmRNA和蛋白水平，这可能是氟中毒学习记忆障碍的机制之一。关键词:氟化物;中枢神经系统;钙/钙调素依赖性蛋白激酶-Ⅱ

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The effect of fluoride on a subunit of calcium/calmodulin-dependent protein kinase- II mRNA and protein expression in central nervous system

Objective To investigate the effect of fluoride on the expression of a subunit of calcium/calmodulin-dependent protein kinase- Ⅱ (α-CaMK Ⅱ ) at both mRNA and protein levels in human neuroblastoma cells were cultured in DMEM with final concentrations of NaF 0(control) ,0.05,0.50,2.00,5.00 mmol/L, respectively, for 48 hours. Then quantitative RT-PCR and Western blot were performed to detect the expression level of α-CaMK Ⅱ P1 (postnatal day 1) pups together with their mothers were randomly divided into three groups. Lactating rats were given drinking water containing NaF at concentrations 0(control) ,2,3 mmol/L. And pups were exposed to NaF through milk. In each group, 8 pups were sacrificed on day 14 after birth. In post-weaning period, another 8 pups in each group were given drinking water with the same dose of fluoride as their mother's 21 day after birth. After then, these pups were killed on day 28, and hippocampus was dissected immediately and Western blot was conducted mRNA and protein levels were decreased. When NaF concentrations were 0,0.05,0.50,2.00,5.00 mmol/L, the mRNA relative ratios of α-CaMKⅡ in SY5Y cells were 1.00 ± 0.00,0.77 ± 0.18,0.40 ± 0.11,0.22 ± 0.06 and 0.15 ± 0.03, and protein levels of α-CaMK Ⅱ were 100.00 ± 0.00,76.17 ± 2.08,59.16 ± 2.12,48.52 ± 2.71 and 43.51 ± 2.57, any mmol/L group, hippocampus α-CaMK Ⅱ protein levels on day 14 and 28(75.02 ± 2.88,73.83 ± 3.88 and 81.00 ± 2.54,45.70 ± 2.34) were significantly lower than that of control groups(100.00 ± 0.00,100.00 ± 0.00, all P < 0.01). In 3 mmol/L group, hippocampus α-CaMKⅡ protein level on day 28 was lower than that of 2 mmol/L group (P < 0.01). Conclusion Fluoride can decrease mRNA and protein levels of α-CaMK Ⅱ in nerve cells and hippocampus, which may be one of the mechanisms of learning and memory impairment by fluorosis. Key words: Fluoride; Central nervous system; Calcium/calmodulin-dependent protein kinase- Ⅱ

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来源期刊

中华地方病学杂志我国对人类健康危害特别严重的地方性疾病：克山病、大骨节病、碘缺乏病、地方性氟中毒、地方性砷中毒、鼠疫、布鲁氏菌病、寄生虫、新冠肺炎等疾病，同时还报道多发性自然疫源性疾病。

CiteScore

1.60

自引率

0.00%

发文量

8714

期刊介绍： The Chinese Journal of Endemiology covers predominantly endemic diseases threatening health of the people in the areas affected by the diseases including Keshan disease, Kaschin-Beck Disease, iodine deficiency disorders, endemic fluorosis, endemic arsenism, plague, epidemic hemorrhagic fever, brucellosis, parasite diseases and the diseases related to local natural and socioeconomic conditions; and reports researches in the basic science, etiology, epidemiology, clinical practice, control as well as multidisciplinary studies on the diseases.