伊朗西北部多药耐药铜绿假单胞菌分离株的OXA-10和OXA-2 ESBLs

S. Farajnia
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引用次数: 2

摘要

广谱β-内酰胺酶(ESBLs)的产生是铜绿假单胞菌多药耐药的共同机制,但不同ESBLs的频率在世界不同地区可能存在显著差异。本研究旨在调查伊朗西北部大不里士地区铜绿假单胞菌临床分离株中OXA-2/OXA-10型ESBLs和1类整合子的流行情况。共有110株铜绿假单胞菌进入研究。采用纸片扩散法测定药敏。采用联合圆盘法确认ESBL的产生,采用聚合酶链反应检测OXA-2/OXA-10 β -内酰胺酶和1类整合子。药敏试验结果显示,对氨曲南(82%)和头孢吡肟(77.3%)的耐药率最高,对亚胺培南(71%)、美罗培南(66.4%)和哌拉西林/他唑巴坦(37.3%)的耐药率最高。在联合圆盘试验中,68株(61.8%)为ESBL产生菌。PCR分析显示,47株(42.7%)菌株携带1类整合子,其中22株(32.3%)含有blaOXA-10基因,7株(10.3%)含有blaOXA-2基因。综上所述,研究区OXA-10型和OXA-2型ESBLs感染率较高,其中亚胺培南和美罗培南对铜绿假单胞菌的抑菌活性最高。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
OXA-10 and OXA-2 ESBLs among multidrug-resistant Pseudomonas aeruginosa isolates from North West of Iran
Production of Extended spectrum β-lactamases (ESBLs) is a common mechanism of resistance in multidrug- Pseudomonas aeruginosa, but the frequency of different ESBLs may vary significantly in different parts of the world. The aim of this study was to investigate the prevalence of OXA-2/OXA-10 type ESBLs and class 1 integron among clinical isolates of P. aeruginosa in Tabriz, North West of Iran. A total of 110 P. aeruginosa isolates was entered in the study. Antibiotic susceptibility was determined by disk diffusion method. Production of ESBL was confirmed by combined disc method, and polymerase chain reaction was used for detection of OXA-2/OXA-10 beta-lactamases and class 1 integrons. Antibiotic susceptibility tests revealed that the highest resistance rate was against aztreonam (82%) and cefepime (77.3%), whereas the highest susceptibility was to imipenem (71%), meropenem (66.4%) and piperacillin/tazobactam (37.3%). In combined disc test, 68 isolates (61.8%) were ESBL producers. PCR analysis showed that 47 (42.7%) isolates carried class 1 integron, among them 22 (32.3%) contained blaOXA-10 and 7 (10.3%) contained blaOXA-2 genes. In conclusion, high prevalence of OXA-10 and OXA-2 type ESBLs were detected in the study region and that imipenem and meropenem were the most active agents against P. aeruginosa isolates.
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