Lívia Maria Soares Nobre, Marina Helena da Silva Lopes, Juliana Geraix, Aurilene Gomes Cajado, Jussara Matyelle Rodrigues Silva, Lyanna Rodrigues Ribeiro, Rosemayre Souza Freire, Diane Isabelle Magno Cavalcante, Deysi Viviana Tenazoa Wong, Ana Paula Negreiros Nunes Alves, Roberto César Pereira Lima-Júnior
{"title":"副益生菌粪肠球菌 EC-12 可预防伊立替康诱发的小鼠肠道粘膜炎的发生。","authors":"Lívia Maria Soares Nobre, Marina Helena da Silva Lopes, Juliana Geraix, Aurilene Gomes Cajado, Jussara Matyelle Rodrigues Silva, Lyanna Rodrigues Ribeiro, Rosemayre Souza Freire, Diane Isabelle Magno Cavalcante, Deysi Viviana Tenazoa Wong, Ana Paula Negreiros Nunes Alves, Roberto César Pereira Lima-Júnior","doi":"10.1016/j.lfs.2022.120445","DOIUrl":null,"url":null,"abstract":"<p><strong>Aims: </strong>This study tested the protective effect of purified paraprobiotic Enterococcus faecalis (EC-12) and an E. faecalis-based formulation (Med LanS) on irinotecan-induced intestinal mucositis murine model.</p><p><strong>Main methods: </strong>C57BL/6 male mice received saline, irinotecan (75 mg/Kg, i.p.), EC-12 (0.3, 1, or 3 × 10<sup>7</sup> CFU/Kg, p.o.) + irinotecan or Med Lan-S (3 × 10<sup>7</sup> CFU/Kg, p.o.) + irinotecan. Body mass variation was assessed daily, and blood samples were collected for evaluating bacteremia and leukocyte count. The ileum was harvested for myeloperoxidase assay, histopathology, quantitative PCR, and immunofluorescence for macrophages (F4/80), TLR4, and IL-18 binding protein (IL-18BP).</p><p><strong>Key findings: </strong>The best therapeutic strategy was EC-12 administration at 3 × 10<sup>7</sup> CFU/Kg, starting 1 week before irinotecan. EC-12 and Med Lan-S did not prevent the irinotecan-induced body mass loss or leukopenia but attenuated the neutrophil infiltration in the intestine and increased the villus/crypt ratio (P < 0.05). Additionally, EC-12 and Med Lan-S reduced the mRNA expression of Cldn-2, Ocln, and Tlr4 versus the irinotecan group (P < 0.05). Irinotecan also augmented the expression of Il-18, IL-18BP, the immunofluorescence of F4/80, and TLR4, while only EC-12 prevented the expression of all these markers. Remarkably, EC-12 and Med Lan inhibited the irinotecan-induced bacterial translocation to the blood.</p><p><strong>Significance: </strong>Paraprobiotic E. faecalis EC-12 prevents the development of intestinal mucositis by downregulating the inflammatory response. Med Lan-S also protects from mucositis. Possibly, the complexity of the formulation accounts for an innate immune-driven protective mechanism.</p>","PeriodicalId":45010,"journal":{"name":"Violence and Gender","volume":"4 1","pages":"120445"},"PeriodicalIF":1.5000,"publicationDate":"2022-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Paraprobiotic Enterococcus faecalis EC-12 prevents the development of irinotecan-induced intestinal mucositis in mice.\",\"authors\":\"Lívia Maria Soares Nobre, Marina Helena da Silva Lopes, Juliana Geraix, Aurilene Gomes Cajado, Jussara Matyelle Rodrigues Silva, Lyanna Rodrigues Ribeiro, Rosemayre Souza Freire, Diane Isabelle Magno Cavalcante, Deysi Viviana Tenazoa Wong, Ana Paula Negreiros Nunes Alves, Roberto César Pereira Lima-Júnior\",\"doi\":\"10.1016/j.lfs.2022.120445\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Aims: </strong>This study tested the protective effect of purified paraprobiotic Enterococcus faecalis (EC-12) and an E. faecalis-based formulation (Med LanS) on irinotecan-induced intestinal mucositis murine model.</p><p><strong>Main methods: </strong>C57BL/6 male mice received saline, irinotecan (75 mg/Kg, i.p.), EC-12 (0.3, 1, or 3 × 10<sup>7</sup> CFU/Kg, p.o.) + irinotecan or Med Lan-S (3 × 10<sup>7</sup> CFU/Kg, p.o.) + irinotecan. Body mass variation was assessed daily, and blood samples were collected for evaluating bacteremia and leukocyte count. The ileum was harvested for myeloperoxidase assay, histopathology, quantitative PCR, and immunofluorescence for macrophages (F4/80), TLR4, and IL-18 binding protein (IL-18BP).</p><p><strong>Key findings: </strong>The best therapeutic strategy was EC-12 administration at 3 × 10<sup>7</sup> CFU/Kg, starting 1 week before irinotecan. EC-12 and Med Lan-S did not prevent the irinotecan-induced body mass loss or leukopenia but attenuated the neutrophil infiltration in the intestine and increased the villus/crypt ratio (P < 0.05). Additionally, EC-12 and Med Lan-S reduced the mRNA expression of Cldn-2, Ocln, and Tlr4 versus the irinotecan group (P < 0.05). Irinotecan also augmented the expression of Il-18, IL-18BP, the immunofluorescence of F4/80, and TLR4, while only EC-12 prevented the expression of all these markers. Remarkably, EC-12 and Med Lan inhibited the irinotecan-induced bacterial translocation to the blood.</p><p><strong>Significance: </strong>Paraprobiotic E. faecalis EC-12 prevents the development of intestinal mucositis by downregulating the inflammatory response. Med Lan-S also protects from mucositis. Possibly, the complexity of the formulation accounts for an innate immune-driven protective mechanism.</p>\",\"PeriodicalId\":45010,\"journal\":{\"name\":\"Violence and Gender\",\"volume\":\"4 1\",\"pages\":\"120445\"},\"PeriodicalIF\":1.5000,\"publicationDate\":\"2022-05-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Violence and Gender\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1016/j.lfs.2022.120445\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2022/3/2 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q2\",\"JCRName\":\"CRIMINOLOGY & PENOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Violence and Gender","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1016/j.lfs.2022.120445","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2022/3/2 0:00:00","PubModel":"Epub","JCR":"Q2","JCRName":"CRIMINOLOGY & PENOLOGY","Score":null,"Total":0}
Paraprobiotic Enterococcus faecalis EC-12 prevents the development of irinotecan-induced intestinal mucositis in mice.
Aims: This study tested the protective effect of purified paraprobiotic Enterococcus faecalis (EC-12) and an E. faecalis-based formulation (Med LanS) on irinotecan-induced intestinal mucositis murine model.
Main methods: C57BL/6 male mice received saline, irinotecan (75 mg/Kg, i.p.), EC-12 (0.3, 1, or 3 × 107 CFU/Kg, p.o.) + irinotecan or Med Lan-S (3 × 107 CFU/Kg, p.o.) + irinotecan. Body mass variation was assessed daily, and blood samples were collected for evaluating bacteremia and leukocyte count. The ileum was harvested for myeloperoxidase assay, histopathology, quantitative PCR, and immunofluorescence for macrophages (F4/80), TLR4, and IL-18 binding protein (IL-18BP).
Key findings: The best therapeutic strategy was EC-12 administration at 3 × 107 CFU/Kg, starting 1 week before irinotecan. EC-12 and Med Lan-S did not prevent the irinotecan-induced body mass loss or leukopenia but attenuated the neutrophil infiltration in the intestine and increased the villus/crypt ratio (P < 0.05). Additionally, EC-12 and Med Lan-S reduced the mRNA expression of Cldn-2, Ocln, and Tlr4 versus the irinotecan group (P < 0.05). Irinotecan also augmented the expression of Il-18, IL-18BP, the immunofluorescence of F4/80, and TLR4, while only EC-12 prevented the expression of all these markers. Remarkably, EC-12 and Med Lan inhibited the irinotecan-induced bacterial translocation to the blood.
Significance: Paraprobiotic E. faecalis EC-12 prevents the development of intestinal mucositis by downregulating the inflammatory response. Med Lan-S also protects from mucositis. Possibly, the complexity of the formulation accounts for an innate immune-driven protective mechanism.
期刊介绍：
Violence and Gender is the only peer-reviewed journal focusing on the role of gender in the understanding, prediction, and prevention of acts of violence. The Journal is the international forum for the critical examination of biological, genetic, behavioral, psychological, racial, ethnic, and cultural factors as they relate to the gender of perpetrators of violence. Through peer-reviewed research, roundtable discussions, case studies, and other original content, Violence and Gender explores the difficult issues that are vital to threat assessment and prevention of the epidemic of violence. Violence and Gender coverage includes: Alcohol and chemical use/abuse Anthropology, social, and cultural influences Biology and physiology Brain health Brain trauma & injury Early childhood development Environmental influences Gender Genetics Group violence: gang, peer, political, government, and religious Mental health: illnesses, disorders, diseases, and conditions Neuropsychology Neuroscience Paraphilic behavior Parenting and familial influences Peer influences Personality and temperament Predatory behavior & aggression Psychopathy Psychopharmacology School, college/university, and workplace influences Sexuality Spirituality Suicidology Threat assessment warning behaviors Video games, films, television, the Internet, and media Violent fantasies Weapons.
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