马来西亚吉隆坡医院内葡萄球菌感染毒力基因的分子检测——简要报告

S. Masri, R. Hamat, M. Rahman
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引用次数: 0

摘要

耐甲氧西林金黄色葡萄球菌(MRSA)仍然是最具医院传染性的病原体,现在是社区相关感染的新问题。本研究对100例儿科患者的鼻腔样本进行了细菌学和分子检测,以鉴定耐药葡萄球菌生物和检测相关基因。结果显示,在检查的100个鼻标本中,25%为金黄色葡萄球菌,65%为凝固酶阴性葡萄球菌(con), 10%为非葡萄球菌阳性。25株金黄色葡萄球菌中,仅有1株鉴定为MRSA。对MRSA载体菌株进行测序鉴定,发现该菌株具有SCCmecⅲ型、Spa t037型和序列239型,存在sea和fnbA基因。结论MRSA载体菌株可以代表当地流行MRSA克隆的克隆分布。因此,需要对来自许多地区的大量菌株进行研究,以确认在马来西亚流行的主要克隆。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Molecular Detection Of Virulence Gene From Nosocomial Staphylococcus Infection In Hospital Kuala Lumpur, Malaysian-A Brief Report
Methicillin-resistant Staphylococcus aureus (MRSA) remains as the most nosocomial pathogen and is now the emerging problem of community-associated infections. In the present study 100 nasal samples from pediatric patients were subjected to bacteriological and molecular tests for the identification drug resistant Staphylococcus organisms and detection of responsible genes. It revealed that out of 100 nares specimens examined 25% were Staphylococcus aureus, 65% were coagulase-negative Staphylococcus (CONS), and 10% were positive other than Staphylococcus. Among 25 Staphylococcus aureus isolates, only 1 identified as MRSA. The MRSA carrier strain was characterized through sequencing and it was observed that the strains possesses SCCmec type III, Spa type t037 and sequence type 239 with presence of sea and fnbA genes. In conclusion MRSA carrier strain can represent the clonal distribution of locally prevalent MRSA clone. Therefore, a study with large number of strains from many regions is needed to confirm the predominant clones circulating in Malaysia.
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