{"title":"马来西亚吉隆坡医院内葡萄球菌感染毒力基因的分子检测——简要报告","authors":"S. Masri, R. Hamat, M. Rahman","doi":"10.5580/2C48","DOIUrl":null,"url":null,"abstract":"Methicillin-resistant Staphylococcus aureus (MRSA) remains as the most nosocomial pathogen and is now the emerging problem of community-associated infections. In the present study 100 nasal samples from pediatric patients were subjected to bacteriological and molecular tests for the identification drug resistant Staphylococcus organisms and detection of responsible genes. It revealed that out of 100 nares specimens examined 25% were Staphylococcus aureus, 65% were coagulase-negative Staphylococcus (CONS), and 10% were positive other than Staphylococcus. Among 25 Staphylococcus aureus isolates, only 1 identified as MRSA. The MRSA carrier strain was characterized through sequencing and it was observed that the strains possesses SCCmec type III, Spa type t037 and sequence type 239 with presence of sea and fnbA genes. In conclusion MRSA carrier strain can represent the clonal distribution of locally prevalent MRSA clone. Therefore, a study with large number of strains from many regions is needed to confirm the predominant clones circulating in Malaysia.","PeriodicalId":22514,"journal":{"name":"The Internet journal of microbiology","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2012-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Molecular Detection Of Virulence Gene From Nosocomial Staphylococcus Infection In Hospital Kuala Lumpur, Malaysian-A Brief Report\",\"authors\":\"S. Masri, R. Hamat, M. Rahman\",\"doi\":\"10.5580/2C48\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Methicillin-resistant Staphylococcus aureus (MRSA) remains as the most nosocomial pathogen and is now the emerging problem of community-associated infections. In the present study 100 nasal samples from pediatric patients were subjected to bacteriological and molecular tests for the identification drug resistant Staphylococcus organisms and detection of responsible genes. It revealed that out of 100 nares specimens examined 25% were Staphylococcus aureus, 65% were coagulase-negative Staphylococcus (CONS), and 10% were positive other than Staphylococcus. Among 25 Staphylococcus aureus isolates, only 1 identified as MRSA. The MRSA carrier strain was characterized through sequencing and it was observed that the strains possesses SCCmec type III, Spa type t037 and sequence type 239 with presence of sea and fnbA genes. In conclusion MRSA carrier strain can represent the clonal distribution of locally prevalent MRSA clone. Therefore, a study with large number of strains from many regions is needed to confirm the predominant clones circulating in Malaysia.\",\"PeriodicalId\":22514,\"journal\":{\"name\":\"The Internet journal of microbiology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2012-06-12\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"The Internet journal of microbiology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.5580/2C48\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"The Internet journal of microbiology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5580/2C48","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Molecular Detection Of Virulence Gene From Nosocomial Staphylococcus Infection In Hospital Kuala Lumpur, Malaysian-A Brief Report
Methicillin-resistant Staphylococcus aureus (MRSA) remains as the most nosocomial pathogen and is now the emerging problem of community-associated infections. In the present study 100 nasal samples from pediatric patients were subjected to bacteriological and molecular tests for the identification drug resistant Staphylococcus organisms and detection of responsible genes. It revealed that out of 100 nares specimens examined 25% were Staphylococcus aureus, 65% were coagulase-negative Staphylococcus (CONS), and 10% were positive other than Staphylococcus. Among 25 Staphylococcus aureus isolates, only 1 identified as MRSA. The MRSA carrier strain was characterized through sequencing and it was observed that the strains possesses SCCmec type III, Spa type t037 and sequence type 239 with presence of sea and fnbA genes. In conclusion MRSA carrier strain can represent the clonal distribution of locally prevalent MRSA clone. Therefore, a study with large number of strains from many regions is needed to confirm the predominant clones circulating in Malaysia.