在实验感染的雨羊中筛选抗羊痂螨(Psoroptes ovis) Pso o 2抗原的抗体可能无法识别受感染的动物

IF 0.2 4区农林科学 Q4 VETERINARY SCIENCES

Vlaams Diergeneeskundig Tijdschrift Pub Date : 2021-10-29 DOI:10.21825/vdt.v90i5.20903

R. Meyermans, K. Bartley, S. Janssens, S. Burgess, N. Buys

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引用次数: 0

摘要

绵羊疥疮是绵羊健康和福利的重大威胁，是由羊疥螨引起的。通过在皮肤刮痕中鉴定寄生虫来诊断感染，最近使用了针对Pso o 2螨过敏原血清抗体的商用ELISA。然而，人们对ELISA在非英国绵羊群体中的表现知之甚少。本研究以6只雨燕羊为研究对象，对其进行了感染实验。检测病变大小，ELISA检测血清抗Pso o 2和新型Pso- eip -1抗原IgG。尽管所有的羊都有感染的迹象，但有两只羊的血清与Pso o 2没有反应。然而，它们确实对Pso-EIP-1有反应。这表明，使用Pso o 2酶联免疫吸附试验(ELISA)可能无法检测到(Swifter)羊的羊痂，这可能对非英国羊品种的常规筛查有影响。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Screening for antibodies against the sheep scab mite (Psoroptes ovis) Pso o 2 antigen in experimentally infested Swifter sheep may fail to identify affected animals

Sheep scab, caused by Psoroptes ovis mites, represents a significant threat to sheep health and welfare. Infestations are diagnosed by parasite identification in skin scrapings, and more recently with a commercial ELISA against serum antibodies to the Pso o 2 mite allergen. However, little is known about the performance of the ELISA in non-UK sheep populations. In this study, six Swifter sheep were experimentally infested with P. ovis. Lesion sizes were monitored and serum IgG against Pso o 2 and the novel Pso-EIP-1 antigens were measured by ELISA. Although all sheep showed signs of infestation, serum from two animals failed to react with Pso o 2. However, they did react to Pso-EIP-1. This indicates that cases of sheep scab in (Swifter) sheep may remain undetected using the Pso o 2 ELISA, which may have implications for routine screening of non- UK sheep breeds.

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来源期刊

Vlaams Diergeneeskundig Tijdschrift 农林科学-兽医学

CiteScore

0.40

自引率

0.00%

发文量

审稿时长

>36 weeks

期刊介绍： The Vlaams Diergeneeskundig Tijdschrift (ISSN 0303-9021) is a scientific journal that is published bimonthly (six issues per year). It presents mainly clinical topics and addresses itself to two very different readerships: the local Dutch speaking veterinarians in Belgium and the Netherlands, and the international veterinary and biomedical research community. Each issue contains scientific papers either in English, or in Dutch with an English abstract.