Y. Ci, Chun-yang Zhang, Jun Feng, Ai-dong Lang, Chun‐hui Huang, Zhengfan Jiang
{"title":"dATP诱导细胞游离凋亡的光电分析方法","authors":"Y. Ci, Chun-yang Zhang, Jun Feng, Ai-dong Lang, Chun‐hui Huang, Zhengfan Jiang","doi":"10.1039/A900317G","DOIUrl":null,"url":null,"abstract":"A new photoelectric method for analyzing cell-free apoptosis had been developed with ITO (a transparent electrode of indium–tin oxide coated borosilicate) technology. The C5710 mice liver nuclei responded with a negative photoelectric current pulse to white light (200–800nm). During the apoptosis of liver nuclei induced by dATP (deoxyadenosine-5-triphosphate), the photoelectric current showed a dynamic decrease, which was in accordance with the results of fluorescence microscopy and agarose gel electrophoresis. This photoelectric analytical method might provide a rapid and sensitive way to evaluate apoptosis quickly and in a continuous fashion.","PeriodicalId":7814,"journal":{"name":"Analytical Communications","volume":"48 1","pages":"143-145"},"PeriodicalIF":0.0000,"publicationDate":"1999-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"2","resultStr":"{\"title\":\"A photoelectric method for analyzing cell-free apoptosis induced by dATP\",\"authors\":\"Y. Ci, Chun-yang Zhang, Jun Feng, Ai-dong Lang, Chun‐hui Huang, Zhengfan Jiang\",\"doi\":\"10.1039/A900317G\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"A new photoelectric method for analyzing cell-free apoptosis had been developed with ITO (a transparent electrode of indium–tin oxide coated borosilicate) technology. The C5710 mice liver nuclei responded with a negative photoelectric current pulse to white light (200–800nm). During the apoptosis of liver nuclei induced by dATP (deoxyadenosine-5-triphosphate), the photoelectric current showed a dynamic decrease, which was in accordance with the results of fluorescence microscopy and agarose gel electrophoresis. This photoelectric analytical method might provide a rapid and sensitive way to evaluate apoptosis quickly and in a continuous fashion.\",\"PeriodicalId\":7814,\"journal\":{\"name\":\"Analytical Communications\",\"volume\":\"48 1\",\"pages\":\"143-145\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1999-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"2\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Analytical Communications\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1039/A900317G\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Analytical Communications","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1039/A900317G","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
A photoelectric method for analyzing cell-free apoptosis induced by dATP
A new photoelectric method for analyzing cell-free apoptosis had been developed with ITO (a transparent electrode of indium–tin oxide coated borosilicate) technology. The C5710 mice liver nuclei responded with a negative photoelectric current pulse to white light (200–800nm). During the apoptosis of liver nuclei induced by dATP (deoxyadenosine-5-triphosphate), the photoelectric current showed a dynamic decrease, which was in accordance with the results of fluorescence microscopy and agarose gel electrophoresis. This photoelectric analytical method might provide a rapid and sensitive way to evaluate apoptosis quickly and in a continuous fashion.
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