Christine Vestergaard Madsen , Karina Dahl Steffensen , Marianne Waldstrøm , Rikke Fredslund Andersen , Charlotte Hasselholt Søgaard , Ivan Brandslund , Anders Jakobsen
{"title":"卵巢癌患者血小板衍生生长因子基因多态性","authors":"Christine Vestergaard Madsen , Karina Dahl Steffensen , Marianne Waldstrøm , Rikke Fredslund Andersen , Charlotte Hasselholt Søgaard , Ivan Brandslund , Anders Jakobsen","doi":"10.1016/j.cloc.2012.02.020","DOIUrl":null,"url":null,"abstract":"<div><h3>Introduction</h3><p>New reliable and validated markers are desirable in the treatment of ovarian cancer. The platelet-derived growth factor (PDGF) system plays an important role in tumor growth and angiogenesis, and high expression of PDGF/PDGF receptor (PDGFR) has been reported in ovarian cancer. Single nucleotide polymorphisms (SNPs) within a gene may be important for the function of the protein. This had led to the hypothesis that SNPs within the PDGF system could have clinical relevance as prognostic biomarkers in ovarian cancer.</p></div><div><h3>Methods</h3><p>The study included hypothesis-generating patient material from 170 patients with histologically verified epithelial ovarian cancer in which 5 tagging SNPs in the promoter region of the <em>PDGF-AA</em>, <em>PDGF-BB, PDGFR-α,</em> and <em>PDGFR-β</em> genes were analyzed by means of SNaPshot Multiplex (Applied Biosystems, Carlsbad, CA) and sequencing methods. The results were validated in an independent second cohort of 85 patients.</p></div><div><h3>Results</h3><p>In the hypothesis-generating study, survival analyses were made for all 5 SNPs. <em>PDGF-AA 1589 G/T</em> genotype was found to be associated with overall survival in univariate analysis (<em>P</em> = .03), with a clear trend seen in Cox multivariate analysis (<em>P</em> = .12; hazard ratio, 0.75). However, the suggested prognostic importance of <em>PDGF-AA 1589 G/T</em> was not confirmed in the second cohort.</p></div><div><h3>Conclusion</h3><p>Tagging SNPs in the promoter region of the <em>PDGF-AA, PDGF-BB, PDGFR-α</em>, and <em>PDGFR-β</em> genes, as evaluated here, are not likely to be of prognostic importance in patients with ovarian cancer.</p></div>","PeriodicalId":100274,"journal":{"name":"Clinical Ovarian and Other Gynecologic Cancer","volume":"5 1","pages":"Pages 10-16"},"PeriodicalIF":0.0000,"publicationDate":"2012-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.cloc.2012.02.020","citationCount":"2","resultStr":"{\"title\":\"Platelet-Derived Growth Factor Gene Polymorphisms in Patients With Ovarian Cancer\",\"authors\":\"Christine Vestergaard Madsen , Karina Dahl Steffensen , Marianne Waldstrøm , Rikke Fredslund Andersen , Charlotte Hasselholt Søgaard , Ivan Brandslund , Anders Jakobsen\",\"doi\":\"10.1016/j.cloc.2012.02.020\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Introduction</h3><p>New reliable and validated markers are desirable in the treatment of ovarian cancer. The platelet-derived growth factor (PDGF) system plays an important role in tumor growth and angiogenesis, and high expression of PDGF/PDGF receptor (PDGFR) has been reported in ovarian cancer. Single nucleotide polymorphisms (SNPs) within a gene may be important for the function of the protein. This had led to the hypothesis that SNPs within the PDGF system could have clinical relevance as prognostic biomarkers in ovarian cancer.</p></div><div><h3>Methods</h3><p>The study included hypothesis-generating patient material from 170 patients with histologically verified epithelial ovarian cancer in which 5 tagging SNPs in the promoter region of the <em>PDGF-AA</em>, <em>PDGF-BB, PDGFR-α,</em> and <em>PDGFR-β</em> genes were analyzed by means of SNaPshot Multiplex (Applied Biosystems, Carlsbad, CA) and sequencing methods. The results were validated in an independent second cohort of 85 patients.</p></div><div><h3>Results</h3><p>In the hypothesis-generating study, survival analyses were made for all 5 SNPs. <em>PDGF-AA 1589 G/T</em> genotype was found to be associated with overall survival in univariate analysis (<em>P</em> = .03), with a clear trend seen in Cox multivariate analysis (<em>P</em> = .12; hazard ratio, 0.75). However, the suggested prognostic importance of <em>PDGF-AA 1589 G/T</em> was not confirmed in the second cohort.</p></div><div><h3>Conclusion</h3><p>Tagging SNPs in the promoter region of the <em>PDGF-AA, PDGF-BB, PDGFR-α</em>, and <em>PDGFR-β</em> genes, as evaluated here, are not likely to be of prognostic importance in patients with ovarian cancer.</p></div>\",\"PeriodicalId\":100274,\"journal\":{\"name\":\"Clinical Ovarian and Other Gynecologic Cancer\",\"volume\":\"5 1\",\"pages\":\"Pages 10-16\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2012-06-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/j.cloc.2012.02.020\",\"citationCount\":\"2\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Clinical Ovarian and Other Gynecologic Cancer\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1941439012000219\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Clinical Ovarian and Other Gynecologic Cancer","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1941439012000219","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Platelet-Derived Growth Factor Gene Polymorphisms in Patients With Ovarian Cancer
Introduction
New reliable and validated markers are desirable in the treatment of ovarian cancer. The platelet-derived growth factor (PDGF) system plays an important role in tumor growth and angiogenesis, and high expression of PDGF/PDGF receptor (PDGFR) has been reported in ovarian cancer. Single nucleotide polymorphisms (SNPs) within a gene may be important for the function of the protein. This had led to the hypothesis that SNPs within the PDGF system could have clinical relevance as prognostic biomarkers in ovarian cancer.
Methods
The study included hypothesis-generating patient material from 170 patients with histologically verified epithelial ovarian cancer in which 5 tagging SNPs in the promoter region of the PDGF-AA, PDGF-BB, PDGFR-α, and PDGFR-β genes were analyzed by means of SNaPshot Multiplex (Applied Biosystems, Carlsbad, CA) and sequencing methods. The results were validated in an independent second cohort of 85 patients.
Results
In the hypothesis-generating study, survival analyses were made for all 5 SNPs. PDGF-AA 1589 G/T genotype was found to be associated with overall survival in univariate analysis (P = .03), with a clear trend seen in Cox multivariate analysis (P = .12; hazard ratio, 0.75). However, the suggested prognostic importance of PDGF-AA 1589 G/T was not confirmed in the second cohort.
Conclusion
Tagging SNPs in the promoter region of the PDGF-AA, PDGF-BB, PDGFR-α, and PDGFR-β genes, as evaluated here, are not likely to be of prognostic importance in patients with ovarian cancer.
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