用反相高效液相色谱法定量测定印度次大陆天然叶片和体外培养物中的vasaka生物碱

Sayeed Ahmad, Madhukar Garg, E. Tamboli, Y. Kamal, S. Ansari
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引用次数: 4

摘要

背景:本研究旨在通过高效液相色谱梯度法同时定量成熟愈伤组织和粗样品中水仙花碱和水仙花苷的含量,建立一种有效的方案来研究印度次大陆不同地理位置的水仙花叶片中次生代谢物的体外生产。材料与方法:色谱柱为c18反相色谱柱,流速为1.0 mL/min,流速为280 nm,正磷酸乙腈浓度为0.1%,梯度检测。结果:该方法在100 ~ 500 μg/mL范围内线性良好,回归系数r2 = 0.991,精密度高,准确度高,稳健性好。结果清楚地表明,与天然培养物相比,体外培养物中生物碱的浓度显著增加。根据国际协调会议的准确性、精密度、鲁棒性、检出限和定量限准则对所提出的方法进行了验证。结论:所建立的方法可用于瓦西里药材的质量控制和瓦西里素及瓦西里酮的分析。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Quantification of vasaka alkaloids in in vitro cultures and in natural leaves from Indian subcontinents by reversed phase- high performance liquid chromatography
Background: The present study was designed to develop an efficient protocol for studying the enhancement of in vitro production of secondary metabolites in Adhatoda vasica leaves collected from the varied geographical locations of Indian sub-continents by a validated simultaneous high-performance liquid chromatography gradient method for the quantification of vasicine and vasicinone in the developed calli and crude samples. Materials and Methods: The analysis was carried out on a reverse phase C18column using 0.1% of orthophosphoric acid: Acetonitrile in gradient manner and carried out the detection at 280 nm wavelength keeping the flow rate of 1.0 mL/min. Results: The simultaneous method was found linear with regression coefficient r2 = 0.991 in a wide range (100–500 μg/mL) precise, accurate, and robust for quinazoline alkaloids in samples. Results clearly showed a significant increase in the concentration of alkaloids in in vitro cultures as compared to natural ones. The proposed method was validated as per the International Conference on Harmonization guidelines for accuracy, precision, robustness, limit of detection, and limit of quantitation. Conclusion: The developed method was found suitable for quality control of A. vasica and for the analysis of vasicine and vasicinone in any type of sample.
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