Si-si Chen, Zhi-jie Tang, Zhao-ming Cao, Hong-juan Chen, Yi Lian, Xin Hu, H. Lian, Wei-juan Zheng
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In vitro toxicity assessment of atmospheric particulate matter on human lung and hepatic cells with agar membrane-based sampling and exposure strategy
ABSTRACT In vitro toxicology research into the health effects of atmospheric particulate matter (PM) depends on the extraction of PM from filters. Previously, we proposed an efficient PM sampling and extraction method based on low-cost agar membrane and performed an exposure study with RAW264.7 macrophages. Here, we extended the application of this strategy, employing lung and hepatic cells, to validate its reliability and reproducibility. One traditional strategy using polytetrafluoroethylene (PTFE) filter was adopted for comparison. Cytotoxicity and proteomics results showed that the PM extracted by two methods induced comparable toxicity to A549 and BEAS-2B cells, while the PM extracted from the agar membranes induced higher toxicity to HepG2 and HL7702 cells than that from the PTFE filters. The differences in the investigations might be associated with the cell's sensitivity to the extracted suspended particles. This work indicated the importance of assessing PM cytotoxicity from the perspective of extraction methods and cell lines.
期刊介绍:
Environmental Pollutants & Bioavailability is a peer-reviewed open access forum for insights on the chemical aspects of pollutants in the environment and biota, and their impacts on the uptake of the substances by living organisms.
Topics include the occurrence, distribution, transport, transformation, transfer, fate, and effects of environmental pollutants, as well as their impact on living organisms. Substances of interests include heavy metals, persistent organic pollutants, and emerging contaminants, such as engineered nanomaterials, as well as pharmaceuticals and personal-care products as pollutants.